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Concentrations for each biomarker in the healthy and cancer groups are shown. Serum from patients with ovarian cancer showed elevated levels of prolac ...
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Human Cancer Biomarker Panel
Description:
Human Cancer Biomarker Panel
Trade Name:
MILLIPLEX
Background Information:
Millipore now proudly offers the Beadlyte® Human Cancer Biomarker Panel. The Cancer Biomarker Panel includes both a Five-Plex assay, which simultaneously measures five serum biomarkers, Macrophage Migration Inhibitory Factor (MIF), Prolactin, CA-125, Leptin and Osteopontin (OPN), and a Single Plex IGF-II assay. The combination of the six serum biomarkers that discriminate between disease-free and ovarian cancer patients was characterized and validated by Dr. Gil Mor of Yale University. Using a unique algorithm developed by Dr. Mor's Laboratory, the profile of the six biomarkers in circulation was used to detect all stages (I, II, III and IV) of ovarian cancer patients with high sensitivity and accuracy. The assays can be used to quantify these biomarkers in serum, plasma, and cell/tissue culture supernatant samples. The kit is for research use only.
Key Applications:
Multiplexing
Application Notes:
Overnight assay using 50µL 1:6 (5-plex) and 1:20 (1-plex) acid/ethanol treated serum or plasma for IGF-II
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Precision, %:
- Intra Assay <5.9 - 10.4
- Inter Assay <4.7 - 9.1
Therapeutic Areas:
Oncology
Sensitivity:
- MIF: 0.400 pg/mL
- Prolactin : 0.900 ng/mL
- CA-125: 0.012 IU/mL
- Leptin:8.2 pg/mL
- OPN: 33.40 pg/mL
- IGF-II: 0.200 ng/mL
Accuracy:
- MIF: 99.9%
- Prolactin: 102.4%
- CA-125: 99.9%
- Leptin: 97.3%
- OPN: 96.6%
- IGF-II: 96.4%
Standard Curve Range:
- MIF: 0.640 - 10,000 pg/ml
- Prolactin: 0.026 - 400 ng/ml
- CA-125: 0.032 - 500 IU/ml
- OPN: 0.026 - 400 ng/ml
- Leptin: 0.006-100 ng/ml
- IGF-II: 0.700 - 500 ng/ml
Packaging:
Consists of a 5-plex and a 1-plex
Materials Required but Not Delivered:
• Luminex® 100™ or Luminex® 200™ System
• Microbead Sonicator, Catalog # 40-002
• vortex mixer
• variable volume (25-1000µl) pipette tips
• micro centrifuge tubes (1.5 ml)
• timer
• plate shaker
• For cell culture samples containing high biotin, serum, plasma, lavage fluid, tissue homogenates: filtration
system:(Millipore Catalog # MAVM0960r)
• Luminex® Sheath Fluid
• Microbead Sonicator, Catalog # 40-002
• vortex mixer
• variable volume (25-1000µl) pipette tips
• micro centrifuge tubes (1.5 ml)
• timer
• plate shaker
• For cell culture samples containing high biotin, serum, plasma, lavage fluid, tissue homogenates: filtration
system:(Millipore Catalog # MAVM0960r)
• Luminex® Sheath Fluid
Kit Type:
Cancer Kits
Components:
- MSBVN1250 MultiScreenHTS-BV PLates (2 each) and 4 adhesive plastic sealers
- 43-050 Serum and Standard Diluent
- 47-035 Standard 1
- 47-033 Standard 5
- 47-037 Biomarker 1, Quality Controls 1,2
- 47-036 Biomarker 5, Quality controls 1,2
- 42-078 Beads 1
- 42-077 Beads 5
- 44-078 Biotin 1
- 44-077 Biotin 5
- 43-051 Beadlyte Assay Buffer
- 43-052 10X Wash Buffer
- 43-053 IGF-II Activation Buffer
- 43-054 IGF-II Neutralization Buffer
- 45-002 Streptavidin-PE
Analytes Available:
- MIF
- Prolactin
- CA-125
- Leptin
- OPN - Osteopontin
- IGF-II
Quality Assurance:
Tested against ELISAs
Detection Methods:
Luminex xMAP
Cross Reactivity:
Non detectable or negligible within the panel
Kit or Assay Type:
- Cancer Kits
- Multiplex Assays
Assay Type:
Multiplex Assays
Storage Conditions:
1 year at 4°C of earliest expiring reagent