Cell Signaling Buffer Kit

• Bibliography
Close
Bibliography
• Rhyne, P. W., et al. Biotechniques, 35:624-9 (2003). .
• Certificates of Analysis
Close
Certificates of Analysis
Collapse » View All »
• FAQs
Close
FAQs
If I am using a plate with a 96 well format, do I have to use the whole plate? » Answer
No. If it is a strip well plate, remove the wells you do not intend to use. If it is not a strip well plate, tape off any unused wells with lab tape to avoid contamination.

What is the pore size of the 96 well plate? » Answer
The pore size of the 96 well plate is 1.2 microns.

What size are the beads? » Answer
The beads are 5.6 microns.

Can the plates used in the Beadlyte® kits be used with a standard ELISA plate washer? » Answer


What is the difference between the Luminex 100 and the Luminex 200 machine? » Answer
For the most part, one of the differences between the Luminex 100 and the Luminex 200 machines is that a thumbwheel has been installed for probe height adjustment in the 200 versus a hex screw in the 100.

Do I have to dilute my samples in assay buffer? » Answer


What types of samples have been tested in the Beadlyte® kits? » Answer
Plasma, serum, tissue culture media, lavage fluid, urine and breast milk have all been used in the Cytokine/Chemokine kits. Cell lysates have been used in the Cell Signaling Kits.

Can I use Upstate's Beadlyte® products with a BioPlex® machine? » Answer


In regards to the software, what is the doublet discriminator gate? » Answer
The doublet discriminator gate is automatically set at 8,000-13,500 and will exclude doublets or other larger bead aggregates.

In regards to the software, what is the CAL1 setting? » Answer
The CAL1 setting sets the voltage of the photomultiplier tube (PMT) for the laser that detects bead colors and defines the bead number.

In regards to the software, what is the CAL2 setting? » Answer
The CAL2 setting sets the voltage of the photomultiplier tube (PMT) for the laser that detects the reporter.

In regards to the software, are there any advantages to a high calibration? » Answer
Experiments using Beadlyte® cytokine detection systems have shown that the high calibration does not increase sensitivity. Although the MFI values are higher, the background also increases.

In regards to the software, are there any advantages of reading a plate with a high RP1 value? » Answer
Reading a plate using “high” RP1 targets may provide increased detection in some assays. If using the higher calibration settings, we recommend that the assay first be read based on the low target value, then re-read based on the high target value. This will prevent the loss of data that may be in the high range of the assay.

How does the Beadlyte® system work? » Answer


Collapse » View All »

Product Support

• Custom Services Quote
• Bulk Services Quote
• Technical Service
• Customer Service