Ordering Information
- : 48-610MAG
- : 1 kit
Product Images
Multiplex analysis of Daudi and HeLa cells treated with IL-4 or IFNalpha.
Immunoprecipitation/Western Blot analysis of multiplexed STAT analytes.
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MILLIPLEXMAP STAT Cell Signaling Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay
Description:
MILLIPLEX® MAP STAT Cell Signaling Magnetic Bead 5-Plex Kit, 48-610MAG
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Product Overview:
The STAT (Signal Transducers and Activators of Transcription) family, consisting of seven mammalian members, regulates many aspects of growth, survival and differentiation in cells. The extracellular binding of cytokines, hormones, growth factors and neurotransmitters to receptors induces phosphorylation of the intracellular Janus kinases (JAKs) that, in turn, phosphorylate STAT proteins at conserved tyrosine residues, activating SH2-mediated dimerization followed by translocation to the nucleus. STAT dimers then bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes.
Applications:
This MILLIPLEX® MAP STAT Cell Signaling Magnetic Bead 5-Plex Kit is a Cell Signaling multiplex assay. The analytes available for this multiplex kit are: STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/699), STAT6 (Tyr641).
Key Applications:
Multiplexing
Application Notes:
• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Configuration:
Premixed
Panel Type:
Cell Signaling
Storage Conditions:
Recommended storage for kit components is 2 - 8°C.
Detection Methods:
- Fluorescent
- Luminex xMAP
Packaging:
96-well plate
Product Name:
MILLIPLEX® MAP STAT Cell Signaling Magnetic Bead 5-Plex Kit, 48-610MAG
Specificity:
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Analytes Available:
STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/699), STAT6 (Tyr641)