MILLIPLEX MAP Total Akt/mTOR Magnetic Bead 11-Plex Kit - Cell Signaling Multiplex Assay

MILLIPLEX MAP Total Akt/mTOR Magnetic Bead 11-Plex Kit, 48-612MAG

MILLIPLEX

1 kit

The Akt signaling pathway, one of the most often dysregulated signaling pathways in cancer, plays an important role in mediating a very broad range of cellular processes such as growth and development, cell cycle, energy homeostasis, and cell survival. Akt, a serine/threonine kinase that phosphorylates over 100 protein substrates, mediating cell survival and proliferation signals, is often itself hyperphosphorylated in various tumor types. Although the Akt gene itself is not known to be mutated in cancers, many of the upstream regulators of Akt, including IR, IRS, PI3K and PTEN, are oncogenes and tumor suppressors. Downstream of Akt, the mammalian Target Of Rapamycin (mTOR) complex is a key regulator of growth and metabolism. As nearly all of the players in the Akt/mTOR signaling pathway are coordinately regulated by phosphorylation, understanding the role of this pathway in normal physiological processes and in diseases such as cancer and diabetes requires the ability to simultaneously measure phosphorylation status of multiple protein targets.

This MILLIPLEX® MAP Total Akt/mTOR Magnetic Bead 11-Plex Kit is a Cell Signaling multiplex assay. The analytes available for this multiplex kit are: Akt (Total), GSK3α (Total), GSK3β (Total), IGF-1R (Total), IR (total), IRS1 (Total), mTOR (Total), p70S6K (Total), PTEN (Total), RPS6 (Total), TSC2 (Total).

Multiplexing

• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 2 (provided).
• The suggested working range of protein concentration for the assay is 15 to 150 ng of total protein/well (25 μL/well at 600 to 6,000 ng/mL).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Cell Signaling

Recommended storage for kit components is 2 - 8°C.

• Intra-Assay: < 8
• Inter-Assay: < 15

Luminex xMAP

96-well plate

MILLIPLEX MAP Total Akt/mTOR Magnetic Bead 11-Plex Kit, 48-612MAG

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Akt (Total), GSK3α (Total), GSK3β (Total), IGF-1R (Total), IR (total), IRS1 (Total), PTEN (Total), RPS6 (Total), TSC2 (Total), mTOR (Total), p70S6K (Total)