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  • : 48-613MAG
  • : 1 kit

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EGFR Profiler Magnetic Bead 8-Plex Analysis of Untreated HeLa Cell Lysates vs. Stimulated A431 Cell Lysates

MILLIPLEX MAP EGFR Profiler Magnetic Bead 8-Plex Kit - Cell Signaling Multiplex Assay

MILLIPLEX MAP EGFR Profiler Magnetic Bead 8-Plex Kit, 48-613MAG
Trade Name:
  • Upstate (Millipore)
1 kit
Product Overview:
Cells respond to their environment in many different ways through intracellular signaling. Signaling through Receptor Tyrosine Kinases (RTKs) often promote increased metabolism and cell growth. Epidermal Growth Factor Receptor (EGFR) is a 175 kDa receptor activated by such ligands as EGF and heregulin. EGF binding induces EGFR autophosphorylation at specific tyrosine residues, promoting the docking of several SH2 domain-containing adaptor proteins to EGFR that, in turn, activate downstream signaling pathways such as MAPK, AKT and STAT. In addition to autophosphorylated tyrosine residues, EGFR contains other phosphorylation sites including phosphothreonine and phosphoserine residues that are involved in crosstalk with other signaling pathways and EGFR down regulation.
The analytes available for this multiplex kit are: EGFR (Thr654), EGFR (Thr669), EGFR (Tyr845), EGFR (Tyr1045), EGFR (Tyr1068), EGFR (Tyr1086), EGFR (Ser1047), EGFR (Total).
Key Applications:
Application Notes:
• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• 1 - 25 µg cell lysate/well (recommended starting concentration is 40 to 1,000 µg protein/mL).
Species Reactivity:
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Conditions:
Recommended storage for kit components is 2 - 8°C.
Product Name:
MILLIPLEX MAP EGFR Profiler Magnetic Bead 8-Plex Kit, 48-613MAG
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Analytes Available:
EGFR (Ser1047), EGFR (Thr654), EGFR (Thr669), EGFR (Total), EGFR (Tyr1045), EGFR (Tyr1068), EGFR (Tyr1086), EGFR (Tyr845)
Panel Type:
Cell Signaling
Precision, %:
  • Intra-Assay (A431): 2-8
  • Intra-Assay (Control Peptide): 2-10
    EGFR total is N/A
  • Inter-Assay (A431): 7-13
  • Intra-Assay (Control Peptide): 4-18
    EGFR total is N/A
Detection Methods:
Luminex xMAP
96-well plate