Ordering Information
- : 48-614MAG
- : 1 kit
Product Images
Multiplex analysis of HepG2 and MCF-7 cells treated with insulin or IGF-1.
Immunoprecipitation/Western Blot analysis of multiplexed analytes in various cells.
see more >)
)
)
)
MILLIPLEX MAP TGFβ Signaling Pathway Magnetic Bead 6-Plex - Cell Signaling Multiplex Assay
Description:
MILLIPLEX MAP TGFβ Signaling Pathway Magnetic Bead 6-Plex, 48-614MAG
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Product Overview:
Cells respond to their environment in many different ways through intracellular signaling. The TGFβ pathway plays a central role in a number of normal cellular processes including proliferation, differentiation, apoptosis, migration, adhesion, immune response and other functions in most cells. Although the TGFβ pathway regulates a wide range of processes, the pathway is fairly simple. TGFβ dimers bind to TGFβ Type II Receptor which recruits and phosphorylates TGFβ Type I Receptor. The Type I Receptor then recruits and phosphorylates SMAD2/3, which then binds to SMAD4 and forms a complex that enters the cell nucleus where it acts as a transcription factor for various genes. TGFβ Receptor activates the SMAD-dependent canonical pathway, as well as SMAD-independent non-canonical pathways, such as PI3K/Akt, ERK, JNK, and p38.
Aberrant TGFβ signaling is involved in the development of multiple diseases, including hematological malignancies such as leukemia, and impaired wound healing, neurodegenerative diseases, developmental disorders and pulmonary hypertension. Loss-of-function mutations promote tumorigenesis by suppressing the immune system and the epithelial mesenchymal transition (EMT), although TGFβ signaling has also been implicated in tumor inhibition. Immune cells such as B cells, T cells, dendritic cells and macrophages secrete TGFβ, which downregulates their proliferation, differentiation and activation by other cytokines. Consequently, modifications in TGFβ signaling have been linked to autoimmune and inflammatory diseases and cancer.
Aberrant TGFβ signaling is involved in the development of multiple diseases, including hematological malignancies such as leukemia, and impaired wound healing, neurodegenerative diseases, developmental disorders and pulmonary hypertension. Loss-of-function mutations promote tumorigenesis by suppressing the immune system and the epithelial mesenchymal transition (EMT), although TGFβ signaling has also been implicated in tumor inhibition. Immune cells such as B cells, T cells, dendritic cells and macrophages secrete TGFβ, which downregulates their proliferation, differentiation and activation by other cytokines. Consequently, modifications in TGFβ signaling have been linked to autoimmune and inflammatory diseases and cancer.
Applications:
This MILLIPLEX® MAP TGFβ Signaling Pathway Magnetic Bead 6-Plex is a Cell Signaling multiplex assay. The analytes available for this multiplex kit are: Akt (Ser473), ERK (Thr185/Tyr187), Smad2 (Ser465/Ser467), Smad3 (Ser423/Ser425), Smad4 (Total), TGFβRII (Total).
Key Applications:
Multiplexing
Application Notes:
• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Panel Type:
Cell Signaling
Storage Conditions:
Recommended storage for kit components is 2 - 8°C.
Precision, %:
- Intra-Assay: < 8
- Inter-Assay: < 12
Detection Methods:
Luminex xMAP
Packaging:
96-well plate
Product Name:
MILLIPLEX MAP TGFβ Signaling Pathway Magnetic Bead 6-Plex, 48-614MAG
Specificity:
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Analytes Available:
Akt (Ser473), ERK (Thr185/Tyr187), Smad2 (Ser465/Ser467), Smad3 (Ser423/Ser425), Smad4 (Total), TGFβRII (Total)