MILLIPLEX MAP Heat Shock Protein Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay

MILLIPLEX MAP Heat Shock Protein Magnetic Bead 5-Plex Kit, 48-615MAG

MILLIPLEX

1 kit

Heat shock proteins (HSPs) are a family of proteins that function as molecular chaperones within the cell, and have many important roles including folding nascent proteins and maintaining proteins in a folded, active state. They can be induced in response to a variety of stresses including heat shock, radiation, and cytotoxic drug exposure, and have increased expression in cancer cells relative to normal cells. A number of these proteins are being investigated as potential targets for cancer therapy, as they can have effects on proliferation, apoptosis, metastasis, and chemotherapy resistance in certain cancers. The levels of some of the family members are coordinately regulated by the transcription factor Heat Shock Factor 1 (HSF1), in response to a variety of cellular stresses. Interestingly, HSP90 inhibitors can disrupt the interaction of HSP90 with HSF1, which can lead to increased expression of HSP family members such as HSP70 and influence the efficacy of treatment. To better understand the roles and regulation of heat shock proteins and the heat shock response in cancer and other physiological processes, there is increased interest in simultaneous analysis of key heat shock proteins in a panel or multiplex format, rather than studying each protein in isolation.

This MILLIPLEX® MAP Heat Shock Protein Magnetic Bead 5-Plex Kit is a Cell Signaling multiplex assay. The analytes available for this multiplex kit are: HSP27 (Total), HSP27 (pS78/pS82), HSP60, HSP70, HSP90α.

Multiplexing

• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• The suggested working range of protein concentration for the assay is 15 to 150 ng of total protein/well (25 μL/well at 600 to 6,000 ng/mL).

Human

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Cell Signaling

Recommended storage for kit components is 2 - 8°C.

Luminex xMAP

96-well plate

MILLIPLEX MAP Heat Shock Protein Magnetic Bead 5-Plex Kit, 48-615MAG

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

HSP27 (Total), HSP27 (pS78/pS82), HSP60, HSP70, HSP90α