Ordering Information
- : 48-618MAG
- : 1 kit
Product Images
Multiplex analysis of MCF-7 cells treated with IGF-1.
Immunoprecipitation/Western Blot analysis of multiplexed analytes in MCF-7 cells.
MILLIPLEX MAP Total/Phospho Akt 48-618MAG - Cell Signaling Multiplex Assay
Description:
MILLIPLEX MAP Total/Phospho Akt Magnetic Bead 2-plex Kit, 48-618MAG
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Product Overview:
The MILLIPLEX® MAP Total/Phospho Akt (48-618MAG) and Total/Phospho ERK (Catalog #48-619MAG) Magnetic bead kits have been developed for the simultaneous detection of phosphorylated Akt (Ser473) and total Akt or phosphorylated ERK1/2 (Thr185/Tyr187) and total ERK in a single well using the Luminex system. These detection assays are rapid, convenient alternatives to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples.
Applications:
This MILLIPLEX® MAP Total/Phospho Akt Magnetic Bead 2-plex Kit is a Cell Signaling multiplex assay. The analytes available for this multiplex kit are: Akt (Total), Akt (Ser473).
Key Applications:
Multiplexing
Application Notes:
• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL)
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL)
Species Reactivity:
- Human
- Mouse
- Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Configuration:
Premixed
Panel Type:
Cell Signaling
Detection Methods:
Luminex xMAP
Packaging:
96-well plate
Product Name:
MILLIPLEX MAP Total/Phospho Akt Magnetic Bead 2-plex Kit, 48-618MAG
Specificity:
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Analytes Available:
Akt (Ser473), Akt (Total)