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10-Plex MAPK/SAPK Magnetic Bead Kit Analysis of Treated Cells
Immunoprecipitation/Western Blot analysis of multiplexed analytes.
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MILLIPLEX MAP MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay
Description:
MILLIPLEX MAP MAPK/SAPK Signaling 10-Plex Kit , 48-660MAG
Trade Name:
MILLIPLEX
Qty/Pk:
Kit
Product Overview:
Cells respond to their environment in various ways through intracellular signaling. Signaling through Receptor Tyrosine kinases (RTKs) often promotes increased metabolism and cell growth. Activation of ERK/MAP kinase is one of the key serine/threonine kinases activated via RTK signaling, promoting increased activity of p70 S6 kinase, MSK1, STATs, CREB, and many other signaling intermediates. Other signaling pathways induced via stress (ex. heat shock/arsenite treatment) or death receptors (ex. TNFα) promote p38, JNK, and p53 signaling pathways.
Applications:
This MILLIPLEX® MAP MAPK/SAPK Signaling 10-Plex Kit is a Cell Signaling multiplex assay. The analytes available for this multiplex kit are: ATF2 (Thr71), Erk (Thr185/Tyr187), HSP27 (Ser78), JNK (Thr183/Tyr185), c-Jun (Ser73), MEK1 (Ser222), MSK1 (Ser212), p38 (Thr180/Tyr182), p53 (Ser15), STAT1 (Tyr707).
Key Applications:
Multiplexing
Application Notes:
• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).
• This assay requires 25 µL diluted cell lysate.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Configuration:
Premixed
Panel Type:
Cell Signaling
Precision, %:
- Intra-Assay: 3% - 10%
- Inter-Assay: 4% - 13%
Detection Methods:
Luminex xMAP
Product Name:
MILLIPLEX MAP MAPK/SAPK Signaling 10-Plex Kit , 48-660MAG
Specificity:
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Analytes Available:
ATF2 (Thr71), Erk (Thr185/Tyr187), HSP27 (Ser78), JNK (Thr183/Tyr185), MEK1 (Ser222), MSK1 (Ser212), STAT1 (Tyr707), c-Jun (Ser73), p38 (Thr180/Tyr182), p53 (Ser15)