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  • : 48-671MAG
  • : 1 kit

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Multiplex analysis of HEK293 cells treated with serum.

MILLIPLEX MAP Total Mitogenesis RTK Magnetic Bead 7-Plex Kit - Cell Signaling Multiplex Assay

Description:
MILLIPLEX MAP Total Mitogenesis RTK Magnetic Bead 7-Plex Kit, 48-671MAG
Trade Name:
  • MILLIPLEX
  • Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
RTKs (Receptor Tyrosine Kinases) are transmembrane proteins that act as receptors for growth factors, neurotrophic factors and other extracellular signaling molecules. Upon binding extracellular ligands, RTKs can undergo receptor dimerization and tyrosine phosphorylation on specific residues in the cytoplasmic tails which then lead to RTK-mediated signal transduction. Of the greater than 500 known protein kinases in the human genome, approximately 60 are members of the RTK family. They are initiators of cell signaling networks and play crucial roles in normal physiological processes and disease processes ranging from diabetes to cancer.
Applications:
The analytes available for this multiplex kit are: EGFR (Total), ErbB2/HER2 (Total), ErbB3/HER3 (Total), ErbB4/HER4 (Total), IR (total), c-Met/HGFR (Total), IGF-1R (Total).
Key Applications:
Multiplexing
Application Notes:
• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Conditions:
Recommended storage for kit components is 2 - 8°C.
Product Name:
MILLIPLEX MAP Total Mitogenesis RTK Magnetic Bead 7-Plex Kit, 48-671MAG
Specificity:
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Analytes Available:
EGFR (Total), ErbB2/HER2 (Total), ErbB3/HER3 (Total), ErbB4/HER4 (Total), IGF-1R (Total), IR (total), c-Met/HGFR (Total)
Configuration:
Premixed
Panel Type:
Cell Signaling
Precision, %:
  • Intra-Assay: < 11
  • Inter-Assay: < 9
Detection Methods:
Luminex xMAP
Packaging:
96-well plate