Ordering Information
- : 48-673MAG
- : 1 kit
Product Images
Multiplex analysis of Huvec, HFF-1 and THP-1 cells treated with serum.
Percent Cross-reactivity of individual RTK recombinant protein.
MILLIPLEX MAP Total Angiogenesis RTK Magnetic Bead 10-Plex Kit - Cell Signaling Multiplex Assay
Description:
MILLIPLEX® MAP Total Angiogenesis RTK Magnetic Bead 10-Plex Kit, 48-673MAG
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Product Overview:
RTKs (Receptor Tyrosine Kinases) are transmembrane proteins that act as receptors for growth factors, neurotrophic factors and other extracellular signaling molecules. Upon binding extracellular ligands, RTKs can undergo receptor dimerization and tyrosine phosphorylation on specific residues in the cytoplasmic tails which then lead to RTK-mediated signal transduction. Of the greater than 500 known protein kinases in human genome, approximately 60 are members of the RTK family. They are central components of cell signaling networks and play crucial roles in normal physiological processes and disease processes ranging from diabetes to cancer.
Applications:
This MILLIPLEX® MAP Total Angiogenesis RTK Magnetic Bead 10-Plex Kit is a Cell Signaling multiplex assay. The analytes available for this multiplex kit are: c-Kit (Total), Flt3 (Total), M-CSFR (Total), PDGFRα (Total), PDGFRβ (Total), Tie1 (Total), Tie2 (Total), VEGF R1 (Total), VEGF R2 (Total), VEGF R3 (Total).
Key Applications:
Multiplexing
Application Notes:
• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
• This assay requires 25 µL diluted cell lysate per well.
• This kit must be run using Assay Buffer 1 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Panel Type:
Cell Signaling
Storage Conditions:
Recommended storage for kit components is 2 - 8°C.
Detection Methods:
Luminex xMAP
Packaging:
96-well plate
Product Name:
MILLIPLEX® MAP Total Angiogenesis RTK Magnetic Bead 10-Plex Kit, 48-673MAG
Specificity:
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.