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MILLIPLEX MAP 8-Plex Multi-Pathway Signaling Kit, Phosphoprotein
Description:
MILLIPLEX MAP 8-Plex Multi-Pathway Signaling Kit, Phosphoprotein
Trade Name:
MILLIPLEX
Qty/Pk:
100 assays
Product Overview:
8-plex Multi-Pathway Signaling kit, phosphoprotein, is used to detect changes in phosphorylated Erk/MAP kinase 1/2 (Thr185/Tyr187), STAT3 (Ser727), JNK (Thr183/Tyr185), p70 S6 kinase (Thr412), IkBalpha (Ser32), STAT5A/B (Tyr694/699), CREB (Ser133), and p38 (Thr180/Tyr182) in cell lysates using the Luminex® system. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for 100 individual assays.
Key Applications:
Multiplexing
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Configuration:
Premixed
Components:
- 8-plex Multi-Pathway-Phosphoprotein, beads
- 8-plex Multi-Pathway-Phosphoprotein, biotin
- Lysis Buffer (Cat.# 43-040)
- HeLa Cell lysate: HS/Ars (Cat.# 47-211)
- HeLa Cell lysate: TNFalpha (Cat.# 47-212)
- A431 Cell lysate: EGF (Cat.# 47-210)
- HeLa Cell lysate: unstimulated (Cat.# 47-205)
- Amplification Buffer (Cat.# 43-024A)
- Assay buffer 2 (Cat. # 43-041)
- 96-well Filter Plate (Cat.# MX-PLATE)
- Streptavidin-Phycoerythrin (Cat.# 45-001D)
- Empty mixing vials
Brand Family:
Upstate
Premixed or Single Plex:
Yes
Panel Type:
Cell Signaling
Detection Methods:
- Fluorescent
- Luminex xMAP
Array Type:
Antibody Arrays
Product Name:
MILLIPLEX MAP 8-Plex Multi-Pathway Signaling Kit, Phosphoprotein
Materials Required but Not Delivered:
Microbead Sonicator (Catalogue No. 40-002)
MultiScreenHTS Vacuum Manifold (Catalogue No. MSVMHTS00)
Luminex® 200 IS System (Catalogue No. 40-009)
MultiScreenHTS Vacuum Manifold (Catalogue No. MSVMHTS00)
Luminex® 200 IS System (Catalogue No. 40-009)
Analytes Available:
CREB (Ser133), Erk/MAPK 1/2 (Thr185/Tyr187), IκBα (Ser32), JNK/SAPK1 (Thr183/Tyr185), STAT3 (Ser727), STAT5A/B (Tyr694/699), p38/SAPK2A/B (Thr180/Tyr182), p70 S6 Kinase (Thr412)