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MILLIPLEX MAP 7-plex Human T Cell Receptor Signaling Kit - Phosphoprotein
Description:
MILLIPLEX MAP 7-plex Human T Cell Receptor Signaling Kit - Phosphoprotein
Trade Name:
MILLIPLEX
Qty/Pk:
1 kit
Product Overview:
7-plex T Cell Receptor Signaling kit - phosphoprotein is used to detect changes in phosphorylated CREB (Ser133), Erk/MAP kinase 1/2 (Thr185/Tyr187), and phosphorylated tyrosine residues on CD3 epsilon chain, Lck, ZAP-70, LAT, and Syk in T cell lysates using the Luminex® System. Although the protein kinase Syk is not part of the T Cell Receptor pathway, it does play a significant role in B Cell Receptor signaling and acts as a negative control for the analysis of T Cell Receptor signaling. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for 100 individual assays.
Key Applications:
Multiplexing
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
UniProt Summary:
FUNCTION: SwissProt: P43403 # Associates with the T-cell antigen receptor zeta chain (CD3Z). Plays a role in lymphocyte activation.
SIZE: 619 amino acids; 69872 Da
SUBUNIT: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity).
TISSUE SPECIFICITY: Expressed in T- and natural killer cells.
DOMAIN: SwissProt: P43403 The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.
PTM: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
DISEASE:SwissProt: P43403 # Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.
SIZE: 619 amino acids; 69872 Da
SUBUNIT: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity).
TISSUE SPECIFICITY: Expressed in T- and natural killer cells.
DOMAIN: SwissProt: P43403 The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.
PTM: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
DISEASE:SwissProt: P43403 # Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.
Configuration:
Premixed
Components:
- 7-plex Human T Cell Receptor phosphoprotein, beads
- 7-plex Human T Cell Receptor phosphoprotein, biotin
- Lysis Buffer (Cat.# 43-040)
- Amplification Buffer (Cat.# 43-024A)
- Streptavidin-Phycoerythrin (Cat.# 45-001D)
- Assay Buffer 2 (Cat.# 43-041)
- Jurkat Cell lysate: unstimulated (Cat.# 47-206) Jurkat T Cell Lysate: C305 (Cat.# 47-201)
- 96-well Filter Plate (Cat.# MX-PLATE)
- Empty mixing vials
Brand Family:
Upstate
Premixed or Single Plex:
Yes
UniProt Number:
Panel Type:
Cell Signaling
Detection Methods:
- Fluorescent
- Luminex xMAP
Packaging:
Kit capacity: 100 Assay Points
Modifications:
Phosphorylation
Gene Symbol:
- MAPK1
- PRKM2
- MAPK2
- p42-MAPK
- p38
- ERK
- P42MAPK
- ERK2
- p40
- PRKM1
- p41
- ERK-2
- ERT1
- p41mapk
Array Type:
Antibody Arrays
Product Name:
MILLIPLEX MAP 7-plex Human T Cell Receptor Signaling Kit - Phosphoprotein
Entrez Gene Number:
Materials Required but Not Delivered:
Microbead Sonicator (Catalogue No. 40-002)
MultiScreenHTS Vacuum Manifold (Catalogue No. MSVMHTS00)
Luminex® 200 IS System (Catalogue No. 40-009)
MultiScreenHTS Vacuum Manifold (Catalogue No. MSVMHTS00)
Luminex® 200 IS System (Catalogue No. 40-009)
Analytes Available:
CD3ε (pan Tyr), CREB (Ser133), Erk/MAPK 1/2 (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr)