Ordering Information
- : 48-690MAG
- : 96 well plate
Product Images
Multiplex analysis of Jurkat cells treated with H2O2
Immunoprecipitation/Western Blot analysis of multiplexed analytes in various cells.
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MILLIPLEX MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex - Cell Signaling Multiplex Assay
Description:
MILLIPLEX® MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex, 48-680MAG
Trade Name:
MILLIPLEX
Qty/Pk:
96 well plate
Product Overview:
The MILLIPLEX® MAP 7-Plex Human T-Cell Receptor (TCR) Magnetic Bead Kit is used to detect changes in phosphorylated CREB (Ser133), Erk/MAP kinase 1/2 (Thr185/Tyr187), and phosphorylated tyrosine residues on CD3 epsilon chain, Lck, ZAP-70, LAT, and Syk in T-cell lysates using the Luminex® system. Although the protein kinase Syk is not part of the T-Cell Receptor pathway, it does play a significant role in B-Cell Receptor signaling and acts as a negative control for the analysis of T-Cell Receptor signaling. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures.
Applications:
This MILLIPLEX® MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex is a Cell Signaling multiplex assay. The analytes available for this multiplex kit are: CD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr).
Key Applications:
Multiplexing
Application Notes:
• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).
• This assay requires 25 µL diluted cell lysate.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Panel Type:
Cell Signaling
Storage Conditions:
Recommended storage for kit components is 2 - 8°C
Precision, %:
- Intra-Assay: 3% - 8%
- Inter-Assay: 4% - 17%
Detection Methods:
Luminex xMAP
Product Name:
MILLIPLEX® MAP T-Cell Receptor Signaling Magnetic Bead Kit 7-Plex, 48-680MAG
Specificity:
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Analytes Available:
CD3ε (pan Tyr), CREB (S133), ERK (Thr185/Tyr187), LAT (pan Tyr), Lck (pan Tyr), Syk (pan Tyr), ZAP-70 (pan Tyr)