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Casein Kinase 2 (CSNK2A1) siRNA/siAb™ Assay Kit
Description:
Casein Kinase 2 (CSNK2A1) siRNA/siAb™ Assay Kit
Trade Name:
- siRNA/siAb
- Upstate (Millipore)
Product Overview:
siRNA/siAb™ Assay Kits provide convenience and economy by combining into one format products that are guaranteed to knock-down the targeted mRNA and detect the level of the targeted protein. The kit's siRNA SMARTpool®s and antibodies have been extensively validated and together with the included positive and negative controls, they make data generation easier and more trusted. Components available separately. Capacity dependent on choice of plate format, see COA.
Species Reactivity:
Human
Application Notes:
To assist researchers in addressing and minimizing potentially confounding issues associated with siRNA-dependent gene silencing.; 5X Universal Buffer is no longer supplied with this kit, however, it can be made using the recipe found here.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CSNK2A1
- CK2A1
- CKII
Components:
- Catalog # M-003475 SMARTpool® CSNK2A1
- Catalog # D-001206-13-01 Non-specific Control Pool (Negative control)
- Catalog# 06-873 Anti-Casein Kinase 2
- Catalog# 12-305 3T3/A31 Cell Lysate
- 5X Universal Buffer no longer supplied (See Application Notes for details.)
Sales Restrictions:
This product is not available for sale in Australia, New Zealand, Singapore, Indonesia, Thailand, The Phillippines, Malaysia, Vietnam, Japan, Korea, and China.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene.
Key Applications:
- RNAi/siRNA/Gene Knockdown
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P68400 # Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.
SIZE: 391 amino acids; 45144 Da
SUBUNIT: Tetramer composed of an alpha chain, an alpha' and two beta chains. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Interacts with RNPS1.
SIMILARITY: SwissProt: P68400 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. & Contains 1 protein kinase domain.
SIZE: 391 amino acids; 45144 Da
SUBUNIT: Tetramer composed of an alpha chain, an alpha' and two beta chains. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Interacts with RNPS1.
SIMILARITY: SwissProt: P68400 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. & Contains 1 protein kinase domain.
Qty/Pk:
1 kit
Brand Family:
Upstate
Host:
Rabbit
RNAi Product Type:
siRNA/siAb Kits
Product Name:
Casein Kinase 2 (CSNK2A1) siRNA/siAb™ Assay Kit