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FAK (PTK2) siRNA/siAb™ Assay Kit
Description:
FAK (PTK2) siRNA/siAb™ Assay Kit
Trade Name:
- siRNA/siAb
- Upstate (Millipore)
Product Overview:
siRNA/siAb™ Assay Kits provide convenience and economy by combining into one format products that are guaranteed to knock-down the targeted mRNA and detect the level of the targeted protein. The kit's siRNA SMARTpool®s and antibodies have been extensively validated and together with the included positive and negative controls, they make data generation easier and more trusted. Components available separately. Capacity dependent on choice of plate format, see COA.
Species Reactivity:
Human
Application Notes:
To assist researchers in addressing and minimizing potentially confounding issues associated with siRNA-dependent gene silencing.; 5X Universal Buffer is no longer supplied with this kit, however, it can be made using the recipe found here.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- PTK2
- pp125FAK
- FAK
- FAK1
- FADK
Components:
- FAK (PTK2) SMARTpool® siRNA reagent (Cat.# M-003164)
- D-001206
- Anti-FAK, clone 4.47 (Cat.# 05-537)
- 3T3/A31 Cell Lysate (Cat.# 12-305)
- 5X Universal Buffer no longer supplied (See Application Notes for details.)
Sales Restrictions:
This product is not available for sale in Australia, New Zealand, Singapore, Indonesia, Thailand, The Phillippines, Malaysia, Vietnam, Japan, Korea, and China.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined.
Key Applications:
- Western Blotting
- RNAi/siRNA/Gene Knockdown
UniProt Summary:
FUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
SIZE: 1052 amino acids; 119233 Da
SUBUNIT: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with SHB (By similarity). Interacts with TGFB1I1.
SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side. Note=Constituent of focal adhesions.
TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
DOMAIN: SwissProt: Q05397 The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL (By similarity). & The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
PTM: Phosphorylated on 6 tyrosine residues upon activation.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
SIZE: 1052 amino acids; 119233 Da
SUBUNIT: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with SHB (By similarity). Interacts with TGFB1I1.
SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side. Note=Constituent of focal adhesions.
TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
DOMAIN: SwissProt: Q05397 The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL (By similarity). & The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
PTM: Phosphorylated on 6 tyrosine residues upon activation.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
Qty/Pk:
1 kit
Brand Family:
Upstate
Host:
Mouse
RNAi Product Type:
siRNA/siAb Kits
Product Name:
FAK (PTK2) siRNA/siAb™ Assay Kit