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TrkA siRNA/siAb™ Assay Kit
Description:
TrkA siRNA/siAb™ Assay Kit
Trade Name:
- siRNA/siAb
- Upstate (Millipore)
Product Overview:
siRNA/siAb™ Assay Kits provide convenience and economy by combining into one format products that are guaranteed to knock-down the targeted mRNA and detect the level of the targeted protein. The kit's siRNA SMARTpool®s and antibodies have been extensively validated and together with the included positive and negative controls, they make data generation easier and more trusted. Components available separately. Capacity dependent on choice of plate format, see COA.
Species Reactivity:
Human
Application Notes:
To assist researchers in addressing and minimizing potentially confounding issues associated with siRNA-dependent gene silencing.; 5X Universal Buffer is no longer supplied with this kit, however, it can be made using the recipe found here.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- NTRK1
- MTC
- Trk-A
- TRK
- TRK1
- TRKA
- p140-TrkA
- DKFZp781I14186
- CIPA
Components:
- TrkA SMARTpool® siRNA reagent (Cat.# M-003159)
- D-001206
- Anti-TrkA (Cat.# 06-574)
- A431 Cell Lysate
- 5X Universal Buffer no longer supplied (See Application Notes for details.)
Sales Restrictions:
This product is not available for sale in Australia, New Zealand, Singapore, Indonesia, Thailand, The Phillippines, Malaysia, Vietnam, Japan, Korea, and China.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date.
Key Applications:
- RNAi/siRNA/Gene Knockdown
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P04629 # Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway.
SIZE: 796 amino acids; 87497 Da
SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 which bridges NTRK1 to NGFR (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity). Note=Endocytosed to the endosomes upon treatment of cells with NGF (By similarity).
TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells; isoform TrkA-I is found in non-neuronal tissues.
PTM: Ligand-mediated auto-phosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent.
DISEASE: SwissProt: P04629 # Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis (CIPA) [MIM:256800]. CIPA is characterized by a congenital insensitivity to pain, anhidrosis (absence of sweating), absence of reaction to noxious stimuli, self-mutilating behavior, and mental retardation. This rare autosomal recessive disorder is also known as congenital sensory neuropathy with anhidrosis or hereditary sensory and autonomic neuropathy type IV or familial dysautonomia type II. & Chromosomal aberrations involving NTRK1 are a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Translocation t(1;3)(q21;q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3'-end of NTRK1; a rearrangement with TPM3 generates the TRK transcript by fusing TPM3 to the 3'-end of NTRK1. & Chromosomal aberrations involving NTRK1 are a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5'-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein.
SIMILARITY: SwissProt: P04629 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 2 Ig-like C2-type (immunoglobulin-like) domains. & Contains 3 LRR (leucine-rich) repeats. & Contains 1 protein kinase domain.
SIZE: 796 amino acids; 87497 Da
SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 which bridges NTRK1 to NGFR (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity). Note=Endocytosed to the endosomes upon treatment of cells with NGF (By similarity).
TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells; isoform TrkA-I is found in non-neuronal tissues.
PTM: Ligand-mediated auto-phosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent.
DISEASE: SwissProt: P04629 # Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis (CIPA) [MIM:256800]. CIPA is characterized by a congenital insensitivity to pain, anhidrosis (absence of sweating), absence of reaction to noxious stimuli, self-mutilating behavior, and mental retardation. This rare autosomal recessive disorder is also known as congenital sensory neuropathy with anhidrosis or hereditary sensory and autonomic neuropathy type IV or familial dysautonomia type II. & Chromosomal aberrations involving NTRK1 are a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Translocation t(1;3)(q21;q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3'-end of NTRK1; a rearrangement with TPM3 generates the TRK transcript by fusing TPM3 to the 3'-end of NTRK1. & Chromosomal aberrations involving NTRK1 are a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5'-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein.
SIMILARITY: SwissProt: P04629 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 2 Ig-like C2-type (immunoglobulin-like) domains. & Contains 3 LRR (leucine-rich) repeats. & Contains 1 protein kinase domain.
Qty/Pk:
1 kit
Brand Family:
Upstate
Host:
Rabbit
Detection Methods:
- Chromogenic
- Chromogenic
RNAi Product Type:
siRNA/siAb Kits
Product Name:
TrkA siRNA/siAb™ Assay Kit
Antibody Type:
Polyclonal Antibody