Ordering Information
- : AB10041
- : 100 µg
- : 0.5 mg/mL
Product Images
Western Blot Analysis:
Representative lot data.
Human fetal brain lysate were resolved by electrophoresis, transferred to PVDF and probed wi...
Western Blot (SNAP ID) Analysis:
Representative lot data.
Human fetal brain lysate was resolved by electrophoresis, transferred to PVDF a...
Anti-UBXN1/SAKS1 Antibody
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | WB, IP | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-UBX domain-containing protein 1 (UBXN1/SAKS1) Antibody | AB10041
Promotional Text:
Specificity:
This antibody recognizes full-length UBX domain-containing protein 1.
Molecular Weight:
~39 kDa
Epitope:
Full Length Protein
Immunogen:
GST-tagged recombinant protein corresponding to full-length human UBX domain-containing protein 1.
Background Information:
UBX domain-containing protein 1 (UBXN1 or SAKS1) is a component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. UBX domain-containing protein 1 is also involved in ubiquitin-proteasome systems.
Species Reactivity:
- Human
- Rat
- Mouse
Species Reactivity Note:
Demonstrated to react with human. mouse and rat.
Application Notes:
Immunoprecipitation Analysis: 20 µg from a previous lot of this antibody was used in immunoprecipitation in 1 mL of mouse brain tissue lysate.
Western Blot (SNAP ID) Analysis: 2 µg/mL from a previous lot detected UBX domain-containing protein 1 in 10 µg of human fetal tissue lysate.
Western Blot (SNAP ID) Analysis: 2 µg/mL from a previous lot detected UBX domain-containing protein 1 in 10 µg of human fetal tissue lysate.
Control:
Human fetal brain tissue lysate
Quality Assurance:
Evaluated by Western Blot in human fetal brain lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected UBX domain-containing protein 1 in 10 µg of human fetal brain lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected UBX domain-containing protein 1 in 10 µg of human fetal brain lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable at 2-8°C for 1 year from date of recepit.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- 2B28
- LOC51035
- SAKS1
- UBXD10
Alternate Names:
- SAPK substrate protein 1
- UBA/UBX 33.3 kDa protein
- UBX domain protein 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunoprecipitation
UniProt Summary:
FUNCTION: Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in ubiquitin-proteasome systems By similarity.
SUBUNIT STRUCTURE: Component of a complex required to couple retrotranslocation, ubiquitination and deglycosylation composed of NGLY1, SAKS1, AMFR, VCP and RAD23B. Interacts with HOMER2 By similarity. Interacts directly with VCP. Binds polyubiquitin.
SUBCELLULAR LOCATION: Cytoplasm By similarity.
SEQUENCE SIMILARITIES: Contains 1 UBA domain.
Contains 1 UBX domain.
SUBUNIT STRUCTURE: Component of a complex required to couple retrotranslocation, ubiquitination and deglycosylation composed of NGLY1, SAKS1, AMFR, VCP and RAD23B. Interacts with HOMER2 By similarity. Interacts directly with VCP. Binds polyubiquitin.
SUBCELLULAR LOCATION: Cytoplasm By similarity.
SEQUENCE SIMILARITIES: Contains 1 UBA domain.
Contains 1 UBX domain.
Product Name:
Anti-UBX domain-containing protein 1 (UBXN1/SAKS1)
Concentration:
0.5 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µg
Format:
Affinity Purified
Host:
Rabbit