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Dot Blot
Anti-phospho-ITCH (pT222)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | DB, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q96J02 # E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response.
SIZE: 903 amino acids; 102803 Da
SUBUNIT: Interacts via its WW domains with DRPLA, NFE2 and CBLC. Interacts ... see more »
SIZE: 903 amino acids; 102803 Da
SUBUNIT: Interacts via its WW domains with DRPLA, NFE2 and CBLC. Interacts ... see more »
Entrez Gene Number:
Entrez Gene Summary
Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologou
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Description:
Anti-phospho-ITCH (pT222)
Background Information:
ITCH is an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. ITCH regulates the transcriptional activity of several transcription factors including the hematopoietic transcription factor NF-E2, and may play an important role in the regulation of immune response. ITCH down-regulates Epstein-Barr virus LMP2A activity in B cell signaling, and also interacts with NOTCH1, OCLN, JUN, and JUNB. ITCH has a wide tissue distribution and is localized within the nucleus and cytoplasm. Two ITCH splice variants have been described; an approximately 103 kDa form as well as a truncated form (~98 kDa) lacking 42 amino acids in the N-terminal half of the protein. ITCH contains a MAP Kinase binding site and its activity is modulated by phosphorylation at multiple threonine residues by JNK1.
Alternate Names:
Itchy homolog E3 ubiquitin protein ligase; AIP4; NAPP1
Specificity:
Human ITCH phosphorylated on threonine 222 (T222).
Key Applications:
- Dot Blot
- Western Blotting
Application Notes:
Western Blot: Recommended Dilution 1 - 5 μg/mL
Dot Blot
Dot Blot
Species Reactivity:
Human
Immunogen:
Synthetic peptide corresponding to a portion of human ITCH encompassing the phosphorylated Threonine at position 222 (PPPpTPRRP).
Modifications:
Phosphorylation
Presentation:
Affinity purified immunoglobulin. Liquid in Tris-citrate/phosphate buffer, pH 7-8, containing 0.1% sodium azide.
Storage Conditions:
Maintain at 2-8°C for up to 12 months from date of receipt
Gene Symbol:
- ITCH
- NAPP1
- dJ468O1.1
- Itch
- AIF4
- AIP4
Antibody Category:
Protein Trafficking
Antibody Sub-Category:
Ubiquitin & Ubiquitin Metabolism
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Affinity Purified
Host:
Rabbit
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody