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Western Blot Analysis:
HeLa cell lysates resolved by electrophoresis, transferred to PVDF, and probed with anti-MSX2 (1:125). Proteins were visua...
Anti-MSX2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-MSX2
Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptided selected from human MSX2.
Molecular Weight:
~29 kDa
Immunogen:
Synthetic human MSX2 peptide, a.a. 147-164.
Background Information:
MSX2, also known as HOX-8, is a homeobox containing transcription factor, which is a member of the MSH family of homeobox proteins, and is expressed at sites of epithelial-mesenchymal interaction during embryogenesis. More specifically, MSX2 has been found to be expressed in the epidermis, hair follicles, and fibroblasts of the developing fetal skin, and is detected as a diffuse cytoplasmic signal in fetal epidermis and portions of the hair follicle and dermis. In adult epidermis, however, MSX2 expression is localized to the nucleus. More recently, when in combination with another homeobox gene, MSX1, MSX2 has been shown to play a critical role in the developmental stages of both neural tube and neural crest, suggesting that both the MSX1 and the MSX2 genes play an important role in organogenesis.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Reacts with human and mouse. Comparison of sequence homology suggests that this antibody may also react with rat; however, reactivity with other species has not been tested.
Application Notes:
Western Blotting: Recommended working dilutions are 1:125 – 1:250.
Quality Assurance:
Routinely evaluated by Western blot on HeLa cell lysates.
Presentation:
Purified rabbit polyclonal in buffer containing PBS with 0.09% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- HOX8
- Hox-8
- MSH
- FPP
- CRS2
- PFM
Alternate Names:
- msh homeo box 2
- msh homeobox 2
- msh homeobox homolog 2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the muscle segment homeobox gene family. The encoded protein is a transcriptional repressor whose normal activity may establish a balance between survival and apoptosis of neural crest-derived cells required for proper craniofacial morphogenesis. The encoded protein may also have a role in promoting cell growth under certain conditions and may be an important target for the RAS signaling pathways. Mutations in this gene are associated with parietal foramina 1 and craniosynostosis type 2. [provided by RefSeq]
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Probable morphogenetic role. May play a role in limb-pattern formation. In osteoblasts, suppresses transcription driven by the osteocalcin FGF response element (OCFRE).
SUBUNIT: Interacts with MINT (By similarity). Interacts with G22P1 (Ku70) and XRCC5 (Ku80).
SUBCELLULAR LOCATION: Nucleus.
DISEASE: Defects in MSX2 are the cause of parietal foramina 1 (PFM1) [MIM:168500]; also known as foramina parietalia permagna (FPP). PFM1 is an autosomal dominant disease characterized by oval defects of the parietal bones caused by deficient ossification around the parietal notch, which is normally obliterated during the fifth fetal month.
DISEASE: Defects in MSX2 are the cause of parietal foramina with cleidocranial dysplasia (PFMCCD) [MIM:168550]; also known as cleidocranial dysplasia with parietal foramina. PFMCCD combines skull defects in the form of enlarged parietal foramina and deficient ossification of the clavicles.
DISEASE: Defects in MSX2 are the cause of craniosynostosis type 2 (CRS2) [MIM:604757]; also known as craniosynostosis Boston-type (CSB). CRS2 is an autosomal dominat disorder characterized by the premature fusion of calvarial sutures. The craniosynostosis phenotype is either fronto-orbital recession, or frontal bossing, or turribrachycephaly, or cloverleaf skull. Associated features include severe headache, high incidence of visual problems (myopia or hyperopia), and short first metatarsals. Intelligence is normal.
SIMILARITY: Belongs to the Msh homeobox family.
SIMILARITY: Contains 1 homeobox DNA-binding domain.
SUBUNIT: Interacts with MINT (By similarity). Interacts with G22P1 (Ku70) and XRCC5 (Ku80).
SUBCELLULAR LOCATION: Nucleus.
DISEASE: Defects in MSX2 are the cause of parietal foramina 1 (PFM1) [MIM:168500]; also known as foramina parietalia permagna (FPP). PFM1 is an autosomal dominant disease characterized by oval defects of the parietal bones caused by deficient ossification around the parietal notch, which is normally obliterated during the fifth fetal month.
DISEASE: Defects in MSX2 are the cause of parietal foramina with cleidocranial dysplasia (PFMCCD) [MIM:168550]; also known as cleidocranial dysplasia with parietal foramina. PFMCCD combines skull defects in the form of enlarged parietal foramina and deficient ossification of the clavicles.
DISEASE: Defects in MSX2 are the cause of craniosynostosis type 2 (CRS2) [MIM:604757]; also known as craniosynostosis Boston-type (CSB). CRS2 is an autosomal dominat disorder characterized by the premature fusion of calvarial sutures. The craniosynostosis phenotype is either fronto-orbital recession, or frontal bossing, or turribrachycephaly, or cloverleaf skull. Associated features include severe headache, high incidence of visual problems (myopia or hyperopia), and short first metatarsals. Intelligence is normal.
SIMILARITY: Belongs to the Msh homeobox family.
SIMILARITY: Contains 1 homeobox DNA-binding domain.
Stem Cell Type:
- Neural Stem Cells
- Mesenchymal Stem Cells
Stem Cell Workflow Stage:
Characterization
Qty/Pk:
100 µg
Format:
Purified
Host:
Rabbit
Product Name:
Anti-MSX2
Concentration:
0.25 mg/ml
Antibody Type:
Polyclonal Antibody