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Western Blot Analysis:
Mouse Heart Cytosol lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-IHH (1:250). Proteins...
Anti-IHH
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, R, Ca, Eq, B | WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-IHH
Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptided selected from mouse IHH.
Molecular Weight:
~45 kDa
Immunogen:
Synthetic mouse IHH peptide, a.a. 146-162.
Background Information:
Indian hedgehog (IHH) is a member of the hedgehog family of signaling molecules that play diverse roles in patterning early embryonic events. The hedgehog family of morphogens are regulators of cell proliferation, differentiation, and cell-cell communication, and have been shown to have important roles in organogenesis, spermatogenesis, stem cell maintenance, and oncogenesis. The Indian hedgehog (IHH) signal has an essential role in inducing hematopoietic tissue during embryogenesis, and is synthesized by chondrocytes and thymic epithelial cells. In addition to the development of hematopoietic cells, IHH is also responsible for regulating the rate of bone growth and cartilage differentiation during skeletal morphogenesis and intestinal epithelium.
Species Reactivity:
- Mouse
- Human
- Rat
- Canine
- Horse
- Bovine
Species Reactivity Note:
Reacts with mouse. Comparison of sequence homology suggests that this antibody may also react with human, rat, canine, bovine, and equine; however, reactivity with other species has not been tested.
Application Notes:
Western Blotting: Recommended working dilutions are 1:250 – 1:400.
Quality Assurance:
Routinely evaluated by Western blot on mouse heart cytosol lysates.
Presentation:
Purified rabbit polyclonal in buffer containing PBS with 0.09% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- BDA1
- HHG-2
- HHG2
Alternate Names:
Indian hedgehog homolog
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).
SUBCELLULAR LOCATION: Indian hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side (By similarity). Note=The N-terminal peptide remains associated with the cell surface (By similarity).
SUBCELLULAR LOCATION: Indian hedgehog protein C-product: Secreted, extracellular space (By similarity). Note=The C-terminal peptide diffuses from the cell (By similarity).
TISSUE SPECIFICITY: In the embryo, detected in the developing gut, the growth zone of cartilage of developing long bones, epithelium and urogenital sinus. In the adult kidney, found in proximal convoluted and proximal straight tubule.
DEVELOPMENTAL STAGE: Detected at 10 dpc in developing gut, at 14.5 days dpc in the cartilage primordium and in the developing urogenital sinus. Expression increases with gestional age in kidney and duodenum, becoming maximal in adulthood.
PTM: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity).
PTM: Cholesterylation is required for N-product targeting to lipid rafts and multimerization (By similarity).
PTM: N-palmitoylation is required for N-product multimerization and full activity (By similarity).
SIMILARITY: Belongs to the hedgehog family.
SUBCELLULAR LOCATION: Indian hedgehog protein N-product: Cell membrane; Lipid-anchor; Extracellular side (By similarity). Note=The N-terminal peptide remains associated with the cell surface (By similarity).
SUBCELLULAR LOCATION: Indian hedgehog protein C-product: Secreted, extracellular space (By similarity). Note=The C-terminal peptide diffuses from the cell (By similarity).
TISSUE SPECIFICITY: In the embryo, detected in the developing gut, the growth zone of cartilage of developing long bones, epithelium and urogenital sinus. In the adult kidney, found in proximal convoluted and proximal straight tubule.
DEVELOPMENTAL STAGE: Detected at 10 dpc in developing gut, at 14.5 days dpc in the cartilage primordium and in the developing urogenital sinus. Expression increases with gestional age in kidney and duodenum, becoming maximal in adulthood.
PTM: The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity (By similarity).
PTM: Cholesterylation is required for N-product targeting to lipid rafts and multimerization (By similarity).
PTM: N-palmitoylation is required for N-product multimerization and full activity (By similarity).
SIMILARITY: Belongs to the hedgehog family.
Stem Cell Type:
Hematopoietic Stem Cells
Stem Cell Workflow Stage:
Characterization
Qty/Pk:
100 µg
Format:
Purified
Host:
Rabbit
Product Name:
Anti-IHH
Concentration:
0.25 mg/mL
Antibody Type:
Polyclonal Antibody