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Staining of Embryonic Mouse Neural Tube and Limb Bud: C indicates cartilage. Note that anti-aggrecan, Catalog Number AB1031, stains cartilage in both...
Western blot: A 13-day
embryonic mouse was
homogenized and analyzed
With and without chondroitinase
treatment of the sample.
Chond...
)
)
)
Anti-Aggrecan
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | IF, IH(P), IP, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
Entrez Gene Number:
Entrez Gene Summary
This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene.
Description:
Anti-Aggrecan
Trade Name:
Chemicon (Millipore)
Background Information:
Aggregating chondroitin sulfate proteoglycan or aggrecan, is one of the most studied proteoglycans. Many individual monomers of aggrecan bind to hyaluronic acid to form an aggregate, the monomer is known as aggrecan. The aggregates are comprised of up to 100 monomers attached to a single chain of hyaluronic acid. The high content of negatively charged polysaccharide chains contributes to the osmotic swelling of cartilage and in combination with intact collagen fibers results in articular cartilage resistance to compressive forces and high tensile strength. Aggrecan is abundant in articular cartilage and compromises 10% of its dry weight.
Key Applications:
- Immunofluorescence
- Immunohistochemistry (Paraffin)
- Immunoprecipitation
- Western Blotting
Application Notes:
Immunohistochemistry(Paraffin):
A previous lot of this antibody was used at 10 μg/mL. See note under Protocol regarding triton X-100 treatments.
Immunoprecipitation:
A previous lot of this antibody was used at 5 μg/ 500 μL of cell membrane extract in PBS with inhibitors and >0.5% triton X-100.
A previous lot of this antibody was used at 10 μg/mL. See note under Protocol regarding triton X-100 treatments.
Immunoprecipitation:
A previous lot of this antibody was used at 5 μg/ 500 μL of cell membrane extract in PBS with inhibitors and >0.5% triton X-100.
Species Reactivity:
Mouse
Species Reactivity Note:
Mouse. Expected to cross react with Human and rat.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Control:
Cartilage, neural tube, and brain tissue.
Presentation:
Rabbit polyclonal antigen affinity purified antibody in PBS. Anti-GST has not been adsorbed. Liquid.
Note: Aggrecan is present in articular cartilage, brain, heart, and other tissues.
Note: Aggrecan is present in articular cartilage, brain, heart, and other tissues.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Gene Symbol:
- ACAN
- MSK16
- SEDK
- CSPCP
- AGCAN
- AGC1
- CSPG1
- CSPGCP
Format Code:
Pur
Alternate Names:
- Cartilage-specific proteoglycan core protein
- Cartilage-specific proteoglycan core protein
- aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122)
- aggrecan proteoglycan
- aggrecan proteoglycan
- large aggregating proteoglycan
Concentration:
0.5 mg/mL
Antibody Type:
Polyclonal Antibody
Specificity:
AB1031 is specific for mouse aggrecan; other species have not been tested.
Quality Assurance:
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected AGGRECAN on 10 μg of Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected AGGRECAN on 10 μg of Mouse Brain lysates.
Immunogen:
GST fusion protein containing amino acids 1177-1326 of mouse aggrecan.
Format:
Affinity Purified
Host:
Rabbit
Antibody Category:
Cell Structure
Antibody Sub-Category:
ECM Proteins
Purification Method:
Immunoaffinity Purified