Ordering Information
- : AB10555
- : 100 µL
Product Images
Anti-Endophilin B1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB | Rabbit | null | Polyclonal Antibody |
Description:
Anti-Endophilin B1 Antibody | AB10555
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Specificity:
This antibody recognizes Endophilin B1.
Molecular Weight:
Approx. 40 kDa
Epitope:
N-terminus
Immunogen:
This antibody is generated from rabbits immunized with a recombinant protein corresponding to residues surrounding the N-terminus region of Endophilin B1.
Background Information:
Endophilin B1 may be required for normal outer mitochondrial membrane dynamics. It is required for coatomer-mediated retrograde transport in certain cells and may recruit other proteins to membranes with high curvature. Additionally, it promotes membrane fusion.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Demonstrated to react with human. Predicted to react with mouse. Canine (80%) and Rat (73%)
Application Notes:
Western Blot (SNAP ID) Analysis: 1/500 to 1/1000 dilution from a previous lot detected Endophilin on 10 µg of HeLa cell lysate.
Control:
HeLa cell lysate
Quality Assurance:
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: : 1/500 to 1/1000 dilution of this antibody detected Endophilin B1 on 10 µg of HeLa cell lysate.
Western Blot Analysis: : 1/500 to 1/1000 dilution of this antibody detected Endophilin B1 on 10 µg of HeLa cell lysate.
Presentation:
Purified rabbit polyclonal in PBS 0.09% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8°C for 1 year from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- Bif-1
- CGI-61
- KIAA0491
- OTTHUMP00000011912
- dJ612B15.2
Alternate Names:
- Bax-interacting factor 1
- SH3 domain-containing GRB2-like protein B1
- SH3-containing protein SH3GLB1
- SH3-domain GRB2-like endophilin B1
- SH3-domain, GRB2-like, endophilin B1
- endophilin B1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion.
SUBUNIT STRUCTURE: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1 By similarity. Homodimer, and heterodimer with SH3GLB2. Binds BAX. Induction of apoptosis augments BAX binding.
SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein By similarity. Mitochondrion outer membrane; Peripheral membrane protein. Note: Association with the Golgi apparatus depends on the cell type By similarity.
TISSUE SPECIFICITY: Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.
DOMAIN: An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.
MISCELLANEOUS: HeLa cells lacking SH3GLB1 show dissociation of outer and inner mitochondrial membrane as well as abnormal mitochondrial morphology. Cells overexpressing SH3GLB1 lacking an N-terminal amphipathic helix show a similar phenotype.
SH3GLB1 binds liposomes and induces formation of tubules from liposomes. SH3GLB1 lacking the N-terminal amphipathic helix fails to induce liposome tubulation.
SEQUENCE SIMILARITIES: Belongs to the endophilin family.
Contains 1 BAR domain.
Contains 1 SH3 domain.
CAUTION: It is uncertain whether Met-1 or Met-4 is the initiator.
Was originally thought to have lysophosphatidic acid acyltransferase activity, but by homology with SH3GL2/endophilin A1 is unlikely to have this activity.
SUBUNIT STRUCTURE: Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1 By similarity. Homodimer, and heterodimer with SH3GLB2. Binds BAX. Induction of apoptosis augments BAX binding.
SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein By similarity. Mitochondrion outer membrane; Peripheral membrane protein. Note: Association with the Golgi apparatus depends on the cell type By similarity.
TISSUE SPECIFICITY: Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.
DOMAIN: An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.
MISCELLANEOUS: HeLa cells lacking SH3GLB1 show dissociation of outer and inner mitochondrial membrane as well as abnormal mitochondrial morphology. Cells overexpressing SH3GLB1 lacking an N-terminal amphipathic helix show a similar phenotype.
SH3GLB1 binds liposomes and induces formation of tubules from liposomes. SH3GLB1 lacking the N-terminal amphipathic helix fails to induce liposome tubulation.
SEQUENCE SIMILARITIES: Belongs to the endophilin family.
Contains 1 BAR domain.
Contains 1 SH3 domain.
CAUTION: It is uncertain whether Met-1 or Met-4 is the initiator.
Was originally thought to have lysophosphatidic acid acyltransferase activity, but by homology with SH3GL2/endophilin A1 is unlikely to have this activity.
Product Name:
Anti-Endophilin B1
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µL
Host:
Rabbit