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Ordering Information

  • : AB10615
  • : 100 µg
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

Anti-PDEF Antibody

Species Reactivity Key Applications Host Format Antibody Type
H ELISA, WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-PDEF Antibody
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes PDEF.
Immunogen:
Synthetic peptide derived from the internal region of human PDEF (prostate-derived Ets factor).
Background Information:
PDEF (prostate-derived Ets factor) is a 37.5-kDa protein that acts as an androgen-independent transcriptional activator for the PSA (prostate specific antigen) promoter. It also interacts directly with the DNA binding domain of androgen receptor and enhances androgen-mediated activation of the PSA promoter. PDEF was shown to have transcript levels 192-fold higher in the peripheral blood of some breast cancer patients in comparison with normal individuals. PDEF mRNA is also frequently over-expressed in human breast tumors as well as in human ovarian tumors. In ovarian tumors, PDEF expression seems to down-modulate malignant potential, and thus provides a rationale to screen for drugs that induce PDEF expression in epithelial ovarian tumors.
Species Reactivity:
Human
Species Reactivity Note:
Human (positive control: LNCaP cells)
Application Notes:
ELISA: 0.1-1μg/mL
Western Blotting: 1-3 μg/mL
Purification Method:
Immunoaffinity purified
Presentation:
This polyclonal antibody is supplied in 400 μL of phosphate buffered saline (pH 7.4) containing 0.1% sodium azide.
Storage Conditions:
Store at -20°C in undiluted aliquots for 1 year from date of shipment.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • SPDEF
  • bA375E1.3
  • PSE
  • PDEF
  • RP11-375E1__A.3
Alternate Names:
Prostate-derived Ets Factor
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • ELISA
  • Western Blotting
Entrez Gene Summary:
PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA (MIM 176820) expression.[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: O95238 # May function as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter. Binds to 5'-GGAT- 3' DNA sequences. May play a role in the regulation of the prostate gland and/or prostate cancer development. Acts as a transcriptional activator for SERPINB5 promoter.
SIZE: 335 amino acids; 37518 Da
SUBUNIT: Interacts with the DNA-binding domain of the androgen receptor. Interacts with NKX3-1.
SUBCELLULAR LOCATION: Nucleus (Probable).
TISSUE SPECIFICITY: Expressed in a very restricted set of primarily hormone-regulated epithelial tissues with particularly high expression in the prostate gland. Significantly lower expression is seen in other hormone regulated tissues such as mammary gland, salivary gland, and ovary. Expressed in prostate carcinoma cells.
SIMILARITY: SwissProt: O95238 ## Belongs to the ETS family. & Contains 1 ETS DNA-binding domain. & Contains 1 PNT (pointed) domain.
Brand Family:
Chemicon
Product Name:
Anti-PDEF Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µg
Format:
Affinity Purified
Host:
Rabbit
Applications:
Detect PDEF using this Anti-PDEF Antibody validated for use in ELISA & WB.

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