Ordering Information
- : AB1244
- : 100 µL
Product Images
Rabbit anti-Cytochrome P450 side chain cleavage enzyme (Catalog Number AB1244) Immunocytochemical localization of P450side chain cleavage enzyme using...
Anti-Cytochrome P450 Side Chain Cleavage Enzyme, a.a. 421-441
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ht, M, Po, R, Rb | IH(P), WB | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-Cytochrome P450 Side Chain Cleavage Enzyme, a.a. 421-441
Trade Name:
Chemicon (Millipore)
Specificity:
Reacts with amino acids 421-441 of rat cytochrome P450 side chain cleavage (P450scc) enzyme.
P450scc enzyme is the rate limiting enzyme controlling steroidogenesis. P450scc is responsible for the conversion of cholesterol to pregnenolone. The enzyme is expressed in all tissues capable of steroidogenesis, including the ovary, adrenal, testis, placenta and glial cells.
P450scc enzyme is the rate limiting enzyme controlling steroidogenesis. P450scc is responsible for the conversion of cholesterol to pregnenolone. The enzyme is expressed in all tissues capable of steroidogenesis, including the ovary, adrenal, testis, placenta and glial cells.
Epitope:
a.a. 421-441
Immunogen:
Amino acids 421-441 on rat cytochrome P450 side chain cleavage (P450scc) enzyme.
Species Reactivity:
- Bovine
- Hamster
- Mouse
- Pig
- Rat
- Rabbit
Application Notes:
Western blot: 1:1,000
Immunohistochemistry on frozen or Bouin's fixed paraffin embedded sections: 1:200 (streptavidin-biotin).
Optimal working dilutions must be determined by end user.
Immunohistochemistry on frozen or Bouin's fixed paraffin embedded sections: 1:200 (streptavidin-biotin).
Optimal working dilutions must be determined by end user.
Presentation:
Rabbit antiserum. Liquid. Contains no preservative
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CYPXIA1
- P450(scc)
- Cholesterol side-chain cleavage enzyme
- Cholesterol desmolase
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system.
Key Applications:
- Immunohistochemistry (Paraffin)
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: Q07973 # Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of 25-hydroxyvitamin D(3) in the presence of adrenodoxin and NADPH-adrenodoxin reductase.
SIZE: 514 amino acids; 58875 Da
SUBCELLULAR LOCATION: Mitochondrion.
SIMILARITY: SwissProt: Q07973 ## Belongs to the cytochrome P450 family.
SIZE: 514 amino acids; 58875 Da
SUBCELLULAR LOCATION: Mitochondrion.
SIMILARITY: SwissProt: Q07973 ## Belongs to the cytochrome P450 family.
Qty/Pk:
100 µL
Brand Family:
Chemicon
Format:
Serum
Host:
Rabbit
Product Name:
Anti-Cytochrome P450 Side Chain Cleavage Enzyme, a.a. 421-441
Antibody Type:
Polyclonal Antibody