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Western Blot Analysis:
Representative lot data.
NIH/3T3 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with...
Western Blot Analysis:
Representative image from a previous lot.
A431 (Lane1), C2C12 lysate (Lane2), HEK293 lysate (Lane 3), Hela lysate (La...
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Anti-Choline Acetyltransferase
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Av, Gp, H, M, Mk, R, Op | IH, WB | Goat | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Choline Acetyltransferase
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes choline acetyltransferase found in cholinergic neurons in brain and central nervous systems.
Molecular Weight:
70 kDa
Immunogen:
Human placental enzyme.
Species Reactivity:
- Avian
- Guinea Pig
- Human
- Mouse
- Monkey
- Rat
- Opossum
Application Notes:
Immunohistochemistry at 1:100. See attached protocol.
Immunoblot at 1:100-1,000. Expected M.W. 68-70 kD.
Optimal working dilutions must be determined by end user.
Immunoblot at 1:100-1,000. Expected M.W. 68-70 kD.
Optimal working dilutions must be determined by end user.
Control:
Rat brain tissue
Purification Method:
Immunoaffinity purified
Presentation:
Affinity Purified immunoglobulin. Liquid in PBS containing 5 mg/mL BSA and 0.2% sodium azide.
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CHAT
- ChAT
- CHOACTase
- CMS1A
- CMS1A2
Alternate Names:
ChAT
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunohistochemistry
- Western Blotting
Entrez Gene Summary:
Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.
UniProt Summary:
FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
SIZE: 748 amino acids; 82568 Da
DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
SIZE: 748 amino acids; 82568 Da
DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
Brand Family:
Chemicon
Product Name:
Anti-Choline Acetyltransferase
Antibody Type:
Polyclonal Antibody
Qty/Pk:
1 mL
Format:
Affinity Purified
Host:
Goat