Product Images
Goat anti-ChAT (Catalog Number AB144P-200UL) staining of rat hypothalamus. Photograph courtesy of Dr. Robert Sloviter, University of Arizona.
Western Blot Analysis:
Representative lot data.
NIH/3T3 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with...
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Anti-Choline Acetyltransferase
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H, Ch, Av, Gp, Op | IC, IH, WB | Goat | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Choline Acetyltransferase
Trade Name:
Chemicon (Millipore)
Specificity:
Choline acetyltransferase found in cholinergic neurons in brain and central nervous systems.
Molecular Weight:
70 kDa
Immunogen:
Human placental enzyme.
Background Information:
Acetylcholine (ACh) is a common neuro-transmitter for motoneurons, preganglionic autonomic neurons, postganglionic para-sympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.
Species Reactivity:
- Mouse
- Rat
- Human
- Chicken
- Avian
- Guinea Pig
- Opossum
Species Reactivity Note:
Rat, mouse, monkey, guinea pig, chicken, opossum, avian and human (western blot).
Application Notes:
Immunohistochemistry:
A previous lot of this antibody was used in immunohistochemistry at 1:100 dilution. See attached protocol.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was used in immunohistochemistry at 1:100 dilution. See attached protocol.
Optimal working dilutions must be determined by end user.
Control:
Rat hypothalamus tissue.
Quality Assurance:
Evaluated by Western Blot on NIH/3T3 lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected ChAT on 10 µg of NIH/3T3 lysates.
Western Blot Analysis:
1:1000 dilution of this antibody detected ChAT on 10 µg of NIH/3T3 lysates.
Purification Method:
Immunoaffinity purified
Presentation:
Purified goat polyclonal in buffer containing PBS containing 5 mg/mL BSA and 0.2% sodium azide.
Storage Conditions:
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
For short term storage (3-4 weeks) maintain at +2-8°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
For short term storage (3-4 weeks) maintain at +2-8°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CHAT
- ChAT
- CHOACTase
- CMS1A
- CMS1A2
Alternate Names:
- Choline acetylase
- acetyl CoA:choline O-acetyltransferase
- choline acetyltransferase
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Western Blotting
Entrez Gene Summary:
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq].
Transcript Variant: This variant (M) contains alternate 5' exon M, uses an upstream start codon, and encodes isoform 2.
Transcript Variant: This variant (M) contains alternate 5' exon M, uses an upstream start codon, and encodes isoform 2.
UniProt Summary:
FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
SIZE: 748 amino acids; 82568 Da
DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
SIZE: 748 amino acids; 82568 Da
DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
Brand Family:
Chemicon
Product Name:
Anti-Choline Acetyltransferase
Concentration:
Variable
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 µL
Format:
Affinity Purified
Host:
Goat