Anti-Stem Cell Factor

Anti-Stem Cell Factor

Chemicon (Millipore)

Reacts with mouse SCF; SCF is highly conserved cross reactivity with rat, human and other species SCF is expected but untested.

30 kDa

Highly pure (>98%) recombinant mouse SCF

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble form is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Mouse

ELISA: To detect mSCF by direct ELISA (using 100 μL/well antibody solution) a concentration of at lease 0.5 μg/mL of this antibody is required. Should allow detection of 0.2 - 0.4 ng/well of recombinant mSCF.

Neutralization: For ½ maximal inhibition (ND50) of the bioogical activity of mSCF (20 ng/mL), a concentration of 0.64 - 0.97 μg/mL is required.

Western blot: 0.1 - 0.2 μg/mL, this dilution should offer a detection limit of 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Optimal working dilutions must be determined by the end user.

Human mast cells

Affinity purified immunoglobulin. Lyophilized from 0.5X PBS pH 7.2. Reconstitute with sterile distilled water.

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile water to a concentration of = 0.2 mg/ml the antibody is stable for at least six weeks at 2-4ºC.

P21583

• NM_000899.3
• NM_003994.4

• KITLG
• SCF
• MGF
• Kitl
• DKFZp686F2250
• SF
• KL-1

SCF; c-kit Ligand; Mast Cell Growth Factor; MGF

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

• ELISA
• Western Blotting
• Neutralizing

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene.

FUNCTION: SwissProt: P21583 # Stimulates the proliferation of mast cells. Able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Mediates also cell-cell adhesion. Acts synergistically with other cytokines, probably interleukins.
SIZE: 273 amino acids; 30899 Da
SUBUNIT: Homodimer, non-covalently linked (Probable).
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein (By similarity). Note=Also exists as a secreted soluble form (isoform 1 only) (By similarity). & Isoform 2: Cell membrane; Single-pass type I membrane protein (By similarity).
DEVELOPMENTAL STAGE: Acts in the early stages of hematopoiesis.
PTM: A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.
SIMILARITY: SwissProt: P21583 ## Belongs to the SCF family.

Hematopoietic Stem Cells

Characterization

Chemicon

Anti-Stem Cell Factor

1 mg/mL

Polyclonal Antibody

50 µg

Affinity Purified

Rabbit