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Ordering Information

  • : AB1504
  • : 200 µL
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

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Western Blotting Analysis:
Representative lot data.
Mouse brain lysate was resolved by electrophoresis, transferred to PVDF membrane...

Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded rat brain tissue was prepared using heat-induced epitope retrieva...

Anti-Glutamate receptor 1

Species Reactivity Key Applications Host Format Antibody Type
R, M, H, Eq WB, IH(P), IP Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Glutamate receptor 1 Antibody
Promotional Text:
Specificity:
This antibody recognizes Glutamate receptor 1 at the cytoplasmic domain.
Molecular Weight:
Observed at ~106 kDa
Epitope:
Cytoplasmic domain
Immunogen:
KLH-conjugated linear peptide corresponding to human Glutamate receptor 1 at the cytoplasmic domain.
Background Information:
Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. GluR1 is believed to mediate calcium influx, and has a vital function in synaptic plasticity related to learning. Reduction in GluR1 has been caused by FMR1 gene mutation and contributes to fragile X syndrome. Changes in expression of GluR1 receptors on dopamine neurons have been correlated with sensitization of drugs of abuse.
Species Reactivity:
  • Rat
  • Mouse
  • Human
  • Horse
Species Reactivity Note:
Demonstrated to react with human, mouse, and rat. Predicted to react with equine and platypus based on 100% sequence homology.
Application Notes:
Immunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect Glutamate receptor 1 (GluR1) in rat brain tissue.
Control:
Mouse brain lysate
Quality Assurance:
Evaluated by Western Blot in mouse brain lysate.

Western Blot Analysis: 1:1000 dilution of this antibody detected Glutamate receptor 1 (GluR1) on 10 µg of mouse brain lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • GRIA1
  • GluR-K1
  • GluR-A
  • GLUH1
  • GLUR1
  • GLURA
  • HBGR1
  • GluR-1
Alternate Names:
  • AMPA 1
  • AMPA-selective glutamate receptor 1
  • Glutamate receptor ionotropic, AMPA 1
  • glutamate receptor, ionotropic, AMPA 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
Entrez Gene Summary:
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
UniProt Summary:
FUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

>SIZE: 906 amino acids; 101506 Da

SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with CACNG2 and RASGRF2 (By similarity).

SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Interaction with CACNG2 promotes cell surface expression (By similarity).

TISSUE SPECIFICITY: Widely expressed in brain.

PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).

SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10) family.

MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.
Product Name:
Anti-Glutamate receptor 1
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 µL
Format:
Affinity Purified
Host:
Rabbit
Applications:
This Anti-Glutamate receptor 1 Antibody is validated for use in WB, IH(P), IP for the detection of Glutamate receptor 1.

Product Resources

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