Ordering Information
- : AB15358
- : 100 µg
- : 1 mg/mL
Product Images
Western Blot Analysis:
Rat brain cytosol lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-Dynamin 1 (1:5,000). P...
Anti-Dynamin 1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, H, Ca | WB | Sheep | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Dynamin 1
Specificity:
This antibody recognizes Dynamin 1 (765-778).
Molecular Weight:
~100 kDa
Epitope:
Internal Domain
Immunogen:
Linear peptide corresponding to amino acids 765-778 of Dynamin 1 conjugated to Dip. Tox.
Background Information:
Dynamin 1, also known as DNM1, is a human gene. This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.
Species Reactivity:
- Rat
- Mouse
- Human
- Canine
Species Reactivity Note:
Human, Mouse, Rat. Predicted to react with dog based on sequence homology.
Control:
Rat Brain Cytosol Lysate.
Quality Assurance:
Routinely evaluated by Western Blot on Rat Brain Cytosol Lysate.
Western Blot Analysis:
1:5,000 of this lot detected Dynamin 1 on 10 µg of Rat Brain Cytosol Lysate.
Western Blot Analysis:
1:5,000 of this lot detected Dynamin 1 on 10 µg of Rat Brain Cytosol Lysate.
Purification Method:
Antigen affinity purified
Presentation:
Sheep polyclonal serum in buffer containing 0.1M Tris-Glycine (pH7.4) 15mM NaCl containing 0.05% NaN3.
Storage Conditions:
Stable for 1 year at 2-8ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- DNM
- EC 3.6.5.5
Alternate Names:
dynamin 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq].
Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region that results in a frameshift and an early stop codon, compared to variant 1. The resulting isoform (2), also known as internal form 2 and the short C-terminal form, is shorter and has a distinct C-terminus, compared to isoform 1.
Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region that results in a frameshift and an early stop codon, compared to variant 1. The resulting isoform (2), also known as internal form 2 and the short C-terminal form, is shorter and has a distinct C-terminus, compared to isoform 1.
UniProt Summary:
FUNCTION: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis.
Catalytic activity GTP + H(2)O = GDP + phosphate.
SUBUNIT STRUCTURE:Interacts with CAV1 and SH3GLB1. Binds SH3GL1, SH3GL2 and SH3GL3 By similarity.
SUBCELLULAR LOCATION:Cytoplasm. Cytoplasm › cytoskeleton. Note= Microtubule-associated.
SEQUENCE SIMILARITY: Belongs to the dynamin family.
Contains 1 GED domain.
Contains 1 PH domain.
Catalytic activity GTP + H(2)O = GDP + phosphate.
SUBUNIT STRUCTURE:Interacts with CAV1 and SH3GLB1. Binds SH3GL1, SH3GL2 and SH3GL3 By similarity.
SUBCELLULAR LOCATION:Cytoplasm. Cytoplasm › cytoskeleton. Note= Microtubule-associated.
SEQUENCE SIMILARITY: Belongs to the dynamin family.
Contains 1 GED domain.
Contains 1 PH domain.
Protein or Enzyme Type:
Neurobiology
Product Name:
Anti-Dynamin 1
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µg
Format:
Affinity Purified
Host:
Sheep