Anti-APG5
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IC, IH | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-APG5
Trade Name:
Chemicon (Millipore)
Specificity:
APG5 [Autophagy-related protein 5, APG5-like, ATG5, Apoptosis-specific protein].
Immunogen:
Synthetic peptide corresponding to a region within the C-terminus of human APG5 protein.
Background Information:
Autophagy is the process by which cells recycle cytoplasm and dispose of excess or defective organelles. This process is suggested to be involved development, differentiation, growth regulation and tissue remodeling in multicellular organisms. APG5 conjugates to APG12 and this conjugate is required for elongation of the isolation membrane to form a complete spherical autophagosome. The APG5 protein may play an important role in the apoptotic process, possibly within the modified cytoskeleton.
Species Reactivity:
Human
Application Notes:
Western blot: 1:200-1:1,000
Immunocytochemistry: 1:200-1:1,000
Immunohistochemistry: 1:200-1:1,000
Optimal working dilutions must be determined by end user.
Immunocytochemistry: 1:200-1:1,000
Immunohistochemistry: 1:200-1:1,000
Optimal working dilutions must be determined by end user.
Purification Method:
Serum
Presentation:
Lyophilized. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any insoluble material. Contains no preservative.
Storage Conditions:
Maintain lyophilized material frozen at -20°C to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1, ACS or better grade) can be added for additional stability.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ATG5
- ASP
- APG5-like
- APG5
- OTTHUMP00000040507
- APG5L
- hAPG5
Alternate Names:
Autophagy-related protein 5, APG5-like, ATG5, Apoptosis-specific protein
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry
UniProt Summary:
FUNCTION: SwissProt: Q9H1Y0 # May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
SIZE: 275 amino acids; 32447 Da
SUBCELLULAR LOCATION: Cytoplasm. Note=Colocalizes with nonmuscle actin.
TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
SIMILARITY: SwissProt: Q9H1Y0 ## Belongs to the ATG5 family.
SIZE: 275 amino acids; 32447 Da
SUBCELLULAR LOCATION: Cytoplasm. Note=Colocalizes with nonmuscle actin.
TISSUE SPECIFICITY: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
SIMILARITY: SwissProt: Q9H1Y0 ## Belongs to the ATG5 family.
Qty/Pk:
50 µL
Brand Family:
Chemicon
Format:
Serum
Host:
Rabbit
Product Name:
Anti-APG5
Antibody Type:
Polyclonal Antibody