Anti-Synapsin I
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R | IC, IH(P), IP, WB | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-Synapsin I
Trade Name:
Chemicon (Millipore)
Specificity:
Synapsin I (Mr of 80,000 and 77,000). Immunolabeling is blocked by preadsorption of antibody with Synapsin I. Synapsin is located only in brain nerve terminal and is an excellent marker for synapses.
Molecular Weight:
77 & 80 kDa
Immunogen:
Synapsin I (mixture of Ia & Ib) purified from bovine brain.
Background Information:
Synapsin I is a member of the synapsin family. Synapsins are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations of the Synapsin I gene may be associated with X linked disorders with primary neuronal degeneration such as Rett syndrome.
Species Reactivity:
- Bovine
- Human
- Mouse
- Rat
Species Reactivity Note:
Human, bovine, rat, and mouse.
Application Notes:
Western blotting:
1:200 of a previous lot was used for labeled Protein A, 1:1,000 dilution for ECL.
Immunocytochemistry:
1:500-1:2,000 dilution of a previous lot was used.
Immunoprecipitation:
5 μL of a previous lot quantitatively immunoprecipitated all synapsin in 200 μg of rat brain.
Optimal working dilutions must be determined by the end user.
1:200 of a previous lot was used for labeled Protein A, 1:1,000 dilution for ECL.
Immunocytochemistry:
1:500-1:2,000 dilution of a previous lot was used.
Immunoprecipitation:
5 μL of a previous lot quantitatively immunoprecipitated all synapsin in 200 μg of rat brain.
Optimal working dilutions must be determined by the end user.
Control:
Mouse hippocampal primary neurons, Mouse brain lysates, PC-12 cells.
Quality Assurance:
Immunohistochemistry(paraffin): Synapsin representative staining pattern/morphology in rat hippocampal neurons. Tissue was pretreated with Citrate pH 6.0, antigen retrieval. A previous lot of this antibody was diluted to 1:1000, using IHC-Select® reagents used with HRP-DAB. Immunoreactivity is seen directly associated with terminal end of neuronal cell body.
IHC-Paraffin Staining With Epitope Retrieval: Rat Hippocampus
IHC-Paraffin Staining With Epitope Retrieval: Rat Hippocampus
Purification Method:
Unpurified
Presentation:
Rabbit polyclonal antiserum in buffer containing no preservatives.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- SYN1
- SYN1a
- SYNI
- SYN1b
Alternate Names:
Synapsin-1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry (Paraffin)
- Immunoprecipitation
- Western Blotting
Entrez Gene Summary:
This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
UniProt Summary:
FUNCTION: SwissProt: P17600 # Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
SIZE: 705 amino acids; 74111 Da
SUBUNIT: Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1 (By similarity).
SUBCELLULAR LOCATION: Cell junction, synapse.
PTM: Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE: SwissProt: P17600 # Defects in SYN1 are a cause of X-linked recessive epilepsy associated with variable learning disabilities and behavior disorders [MIM:300491].
SIMILARITY: SwissProt: P17600 ## Belongs to the synapsin family.
SIZE: 705 amino acids; 74111 Da
SUBUNIT: Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1 (By similarity).
SUBCELLULAR LOCATION: Cell junction, synapse.
PTM: Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE: SwissProt: P17600 # Defects in SYN1 are a cause of X-linked recessive epilepsy associated with variable learning disabilities and behavior disorders [MIM:300491].
SIMILARITY: SwissProt: P17600 ## Belongs to the synapsin family.
Brand Family:
Chemicon
Protein/Isoform Description:
Synapsins are a multi-gene family of neuronal proteins that are the most abundant proteins on synaptic vesicles. Synapsin I is believed to serve as a link between synaptic vesicles and the neuronal cytoskeleton. Phosphorylation of Synapsin I on Ser603 is concomitant with release of neurotransmitters; this phosphorylation site is thought to be specific for CaM Kinase II, however a number of reports indicate that this site is phosphorylated in a Cam Kinase II-independent manner. Loss of Synapsin I from regions of the hippocampus has been observed in brain sections from Alzheimer's disease patients.
Product Name:
Anti-Synapsin I
Concentration:
Variable
Antibody Type:
Polyclonal Antibody
Qty/Pk:
50 µL
Format:
Serum
Host:
Rabbit