Ordering Information
- : AB1554
- : 100 µL
- : Variable
Product Images
Rabbit anti-p75 (Catalog Number AB1554) Rat PC12 cells
Immunocytochemistry: Confocal IF analysis of membrane staining of PC12 cells with anti-NGFR Rabbit antibody (Red). Actin filaments have been labeled ...
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Anti-NGF Receptor p75
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, H, M, R | FC, IC, IF, IH, IH(P), IP, FUNC, WB | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-Nerve Growth Factor Receptor, p75
Trade Name:
Chemicon (Millipore)
Specificity:
p75 nerve growth factor receptor (p75 Neurotrophin receptor, low affinity neurotrophin receptor), extracellular domain.
Molecular Weight:
44.7 kDa
Epitope:
extracellular
Immunogen:
Extracellular fragment generated from sequences from the third exon of mouse p75 (amino acids 43-161).
Background Information:
Nerve Growth Factor Receptor, also termed NGFR p75, p75NTR, or CD271 is the low affinity NGFR (LNGFR) which binds NGF and other neurotrophins including BDNF, NT3, NT4/5 with similar, low affinity. NGFR p75 is a 75 kDa transmembrane glycoprotein (399 a.a.) consisting of an extracellular domain (222 a.a.) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (22 a.a.), and a cytoplasmic domain (155 a.a.). NGFR p75 is mainly expressed in Schwann cells and neurons and in a variety of non neuronal cells. NGFR p75 is necessary for regulating neuronal growth, migration, differentiation and cell death during development of the central and peripheral nervous system. NGFR p75 plays a central role in the regulation of cell number by apoptosis in the developing CNS.
Species Reactivity:
- Chicken
- Human
- Mouse
- Rat
Species Reactivity Note:
Rat and chicken. Predicted to react with 100% mouse and human based on sequence homology. It has been reported that this antibody does not recognize human or rat p75 (See MAB365) by Western blot.
Application Notes:
Western Blot:
1:1,000-1:2,000 dilution of a previous lot was used on western blot.
Immunohistochemistry:
1:50 and 1:300 dilution from a previous lot detected NGF Receptor P75 using IHC-Select Detection with HRP-DAB.
Immunohistochemistry(paraffin):
1:150 dilution of a previous lot was used (Bouin’s fixative). Reactive on paraffin embedded sections.
Flow Cytometry:
1:1,000 dilution of a previous lot was used on flow cytometry.
Blocking Assay (Inhibition):
A previous lot of this antibody bound NGF to cells expressing p75 at 1:1,000 dilution.
Immunocytochemistry:
1:500 dilution of this antibody from a previous lot detected NGF Receptor P75.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation. 1:500 - 1:5,000 dilution of this lot detected NGF Receptor P75 on 10 µg of PC12 cell lysate.
1:1,000-1:2,000 dilution of a previous lot was used on western blot.
Immunohistochemistry:
1:50 and 1:300 dilution from a previous lot detected NGF Receptor P75 using IHC-Select Detection with HRP-DAB.
Immunohistochemistry(paraffin):
1:150 dilution of a previous lot was used (Bouin’s fixative). Reactive on paraffin embedded sections.
Flow Cytometry:
1:1,000 dilution of a previous lot was used on flow cytometry.
Blocking Assay (Inhibition):
A previous lot of this antibody bound NGF to cells expressing p75 at 1:1,000 dilution.
Immunocytochemistry:
1:500 dilution of this antibody from a previous lot detected NGF Receptor P75.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation. 1:500 - 1:5,000 dilution of this lot detected NGF Receptor P75 on 10 µg of PC12 cell lysate.
Control:
Rat PC12 cells.
Quality Assurance:
Immunocytochemistry:
Representative image from a previous lot.
Confolcal fluorescence analysis of PC12 using AB1554 rabbit polyclonal antibody (Red). Actin filaments have been labeled with Alexa Fluor® 488-Phalloidin (Green). Nucleus is stained with DAPI (Blue).
Representative image from a previous lot.
Confolcal fluorescence analysis of PC12 using AB1554 rabbit polyclonal antibody (Red). Actin filaments have been labeled with Alexa Fluor® 488-Phalloidin (Green). Nucleus is stained with DAPI (Blue).
Purification Method:
Unpurified
Presentation:
Rabbit polyclonal serum containing no preservatives.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- NGFR
- p75(NTR)
- TNFRSF16
- Gp80-LNGFR
- CD271
Alternate Names:
- CD271 antigen
- Low affinity neurotrophin receptor p75NTR
- Low-affinity nerve growth factor receptor
- NGF receptor
- low affinity nerve growth factor receptor
- nerve growth factor receptor
- nerve growth factor receptor (TNFR superfamily, member 16)
- p75 ICD
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Immunocytochemistry
- Immunofluorescence
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Immunoprecipitation
- Affects Function
- Western Blotting
Entrez Gene Summary:
Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.
UniProt Summary:
FUNCTION: SwissProt: P08138 # Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
SIZE: 427 amino acids; 45183 Da
SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P08138 Death domain is responsible for interaction with RANBP9.
PTM: N- and O-glycosylated. & O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. & Phosphorylated on serine residues.
SIMILARITY: Contains 1 death domain. & Contains 4 TNFR-Cys repeats.
SIZE: 427 amino acids; 45183 Da
SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P08138 Death domain is responsible for interaction with RANBP9.
PTM: N- and O-glycosylated. & O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. & Phosphorylated on serine residues.
SIMILARITY: Contains 1 death domain. & Contains 4 TNFR-Cys repeats.
Brand Family:
Chemicon
Product Name:
Anti-NGF Receptor p75
Concentration:
Variable
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µL
Format:
Serum
Host:
Rabbit