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Ordering Information
- : AB15664
- : 100 µL
Product Images
Western Blot Analysis:
Cat. AB15664 can detect NUDEL at 1:5000 dilutions in rat brain cytosol Lysate.
Anti-NUDEL
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | WB | Mouse | Serum | Polyclonal Antibody |
Description:
Anti-NUDEL
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes rat NUDEL
Molecular Weight:
38 kDa
Immunogen:
Synthetic Linear Peptide.
Background Information:
NudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NUDEL), also known as NDEL1, is a human gene. This gene encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. This protein plays a role in nervous system development and is linked to LIS-1, the disease gene for a form of lissencephaly, a disorder of cortical development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Species Reactivity:
Rat
Application Notes:
Western Blot: 1:5000L
Control:
brain lysate
Quality Assurance:
Routinely evaluated by immunoblot.
Presentation:
Serum with 0.05% NaN3
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- NUDE2
- Nudel
- Ndel1
Alternate Names:
- NDEL1
- NudE nuclear distribution gene E homolog (A. nidulans)-like 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
a LIS1-interacting protein [RGD]
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL (By similarity).
SUBUNIT: Self-associates. Interacts with DISC1, dynactin, tubulin gamma, KATNA1, KATNB1, microtubules, PCM1, PCNT, and YWHAE. Interacts directly with NEFL and indirectly with NEFH (By similarity). Interacts with dynein and PAFAH1B1.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Centrosome. Spindle. Note=Localizes to the mitotic spindle and to the to the microtubules of the manchette in elongated spermatids (By similarity). Localizes to the interphase centrosome. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes.
PTM: Phosphorylated in mitosis. Can be phosphorylated by CDC2, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE (By similarity).
SIMILARITY: Belongs to the nudE family.
CAUTION: Was originally (Uniprot Ref.4) thought to function as an oligopeptidase (NUDEL-oligopeptidase or endooligopeptidase A) which could regulate peptide levels relevant to brain function.
SUBUNIT: Self-associates. Interacts with DISC1, dynactin, tubulin gamma, KATNA1, KATNB1, microtubules, PCM1, PCNT, and YWHAE. Interacts directly with NEFL and indirectly with NEFH (By similarity). Interacts with dynein and PAFAH1B1.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Centrosome. Spindle. Note=Localizes to the mitotic spindle and to the to the microtubules of the manchette in elongated spermatids (By similarity). Localizes to the interphase centrosome. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes.
PTM: Phosphorylated in mitosis. Can be phosphorylated by CDC2, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE (By similarity).
SIMILARITY: Belongs to the nudE family.
CAUTION: Was originally (Uniprot Ref.4) thought to function as an oligopeptidase (NUDEL-oligopeptidase or endooligopeptidase A) which could regulate peptide levels relevant to brain function.
Qty/Pk:
100 µL
Brand Family:
Chemicon
Format:
Serum
Host:
Mouse
Protein or Enzyme Type:
Neurobiology
Product Name:
Anti-NUDEL
Antibody Type:
Polyclonal Antibody