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Rabbit anti-Phosphotyrosine (Catalog Number AB1599) staining of HB2 (mammary epithelial) cells for EGF receptor or phosphotyrosine before and after ad...
Anti-Phosphotyrosine Antibody | AB1599
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ELISA, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Phosphotyrosine Antibody | AB1599
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Specific for phosphotyrosine. No reaction with phosphoserine, phosphothreonine, serine, threonine, or tyrosine as confirmed by ELISA and Western blot using phosphorylated and unphosphosphorylated proteins.
Immunogen:
A random amino acid polymer containing phosphotyrosine which was conjugated to Keyhole Limpet Hemocyanin.
Modifications:
Phosphorylation
Species Reactivity:
All
Application Notes:
Western blot: 1:5,000 - 1:30,000 using colorimetric detection. Detection with more sensitive systems, such as chemiluminescence, may require higher antibody dilution. At a dilution of 1:1,000 the antibody was easily able to detect 10 ng (0.2 pmol) of a phosphorylated peptide using chromogenic (BCIP/NBT) development.
Slot blot: 1:10,000.
ELISA: 1:30,000 to 1;100,000 for detection of 1 μg/well of phosphotyrosine-containing substrate.
Optimal working dilutions must be determined by end user.
Slot blot: 1:10,000.
ELISA: 1:30,000 to 1;100,000 for detection of 1 μg/well of phosphotyrosine-containing substrate.
Optimal working dilutions must be determined by end user.
Control:
POSITIVE: EGF-stimulated A431 cells.
Presentation:
Affinity purified immunoglobulin in 0.05m Tris with 0.15M NaCl, pH=7.2, and 0.02% sodium azide.
Storage Conditions:
Maintain frozen at -20°C in undiluted aliquots. For working stocks, it is suggested to dilute the stock 20μL with a solution of 1% BSA in TBS (pH 7.4, that has been filtered through a 0.45μm membrane filter) to enhance the full recovery of the antibody from the supplied tube. Avoid repeated freeze/thaw.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Western Blotting
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-Phosphotyrosine Antibody | AB1599
Concentration:
5.0 mg/mL
Antibody Type:
Polyclonal Antibody