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Rabbit anti-Phosphotyrosine (Catalog Number AB1599) staining of HB2 (mammary epithelial) cells for EGF receptor or phosphotyrosine before and after ad...
Anti-Phosphotyrosine
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| A | ELISA, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Phosphotyrosine
Trade Name:
Chemicon (Millipore)
Specificity:
Specific for phosphotyrosine. No reaction with phosphoserine, phosphothreonine, serine, threonine, or tyrosine as confirmed by ELISA and Western blot using phosphorylated and unphosphosphorylated proteins.
Immunogen:
A random amino acid polymer containing phosphotyrosine which was conjugated to Keyhole Limpet Hemocyanin.
Modifications:
Phosphorylation
Species Reactivity:
All
Application Notes:
Western blot: 1:5,000 - 1:30,000 using colorimetric detection. Detection with more sensitive systems, such as chemiluminescence, may require higher antibody dilution. At a dilution of 1:1,000 the antibody was easily able to detect 10 ng (0.2 pmol) of a phosphorylated peptide using chromogenic (BCIP/NBT) development.
Slot blot: 1:10,000.
ELISA: 1:30,000 to 1;100,000 for detection of 1 μg/well of phosphotyrosine-containing substrate.
Optimal working dilutions must be determined by end user.
Slot blot: 1:10,000.
ELISA: 1:30,000 to 1;100,000 for detection of 1 μg/well of phosphotyrosine-containing substrate.
Optimal working dilutions must be determined by end user.
Control:
POSITIVE: EGF-stimulated A431 cells.
Presentation:
Affinity purified immunoglobulin in 0.05m Tris with 0.15M NaCl, pH=7.2, and 0.02% sodium azide.
Storage Conditions:
Maintain frozen at -20°C in undiluted aliquots. For working stocks, it is suggested to dilute the stock 20μL with a solution of 1% BSA in TBS (pH 7.4, that has been filtered through a 0.45μm membrane filter) to enhance the full recovery of the antibody from the supplied tube. Avoid repeated freeze/thaw.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Western Blotting
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Affinity Purified
Host:
Rabbit
Protein/Isoform Description:
Protein Tyrosine phosphorylation was first described in 1979. The development of the Anti-Phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 is the first and best single monoclonal antibody for the detection of tyrosine phosphorylation. It is the most highly cited (75% of related scientific journal articles over the last three years) and validated phosphotyrosine antibody available. It consistently produces high quality, reproducible data, and has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 18 years. In addition to its unparalleled characterization and applicability, 4G10 is well known as one of the most sensitive anti-phosphotyrosines. Now Millipore is excited to announce that we have taken the sensitivity for phosphotyrosine detection to the next level. To improve on something that hundreds have tried and none have succeeded, we pooled 4G10 with the next most highly regarded Anti-phosphotyrosine, clone PY20 to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allows for a greater level of detection on more substrates that even 4G10 alone was capable of.
Product Name:
Anti-Phosphotyrosine
Concentration:
5.0 mg/mL
Antibody Type:
Polyclonal Antibody