Anti-cAMP-Dependent Protein Kinase, Regulatory Subunit II-β, internal
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Mk, Ech | WB, IH | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P31323 # Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
SIZE: 418 amino acids; 46346 Da
SUBUNIT: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules.
TISSUE SPECIFICITY: Four types of regulatory chains are found ... see more »
SIZE: 418 amino acids; 46346 Da
SUBUNIT: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules.
TISSUE SPECIFICITY: Four types of regulatory chains are found ... see more »
Entrez Gene Number:
Entrez Gene Summary
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different
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see more »
Description:
Anti-cAMP-Dependent Protein Kinase, Regulatory Subunit II-β, internal
Alternate Names:
PKA RIIbeta
Specificity:
Recognizes the regulatory subunit IIb of cyclic AMP-dependent protein kinase (PKA RIIbeta). Due to the sequence of the peptide selected, no cross-reactivity is seen with other PKA receptor isoforms.
Key Applications:
- Western Blotting
- Immunohistochemistry
Application Notes:
Western blotting 1:1,000
Immunohistochemistry 1:1,000
Optimal working dilutions must be determined by end user.
Immunohistochemistry 1:1,000
Optimal working dilutions must be determined by end user.
Species Reactivity:
- Human
- Mouse
- Monkey
- Echinoderms
Immunogen:
Synthetic peptide coupled to KLH as an immune potentiator.
Epitope:
Regulatory Subunit II-beta, internal
Presentation:
Peptide-affinity purified immunoglobulin. Liquid at 1 mg/mL containing 0.05% sodium azide and 50% glycerol.
Storage Conditions:
The undiluted antibody solution is stable for approximately 6 months when stored -20°C.
Gene Symbol:
- PRKAR2B
- RII-BETA
- H_RG363E19.2
- WUGSC:H_RG363E19.2
- PRKAR2
Antibody Category:
Cell Signaling
Antibody Sub-Category:
Cytoskeletal Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Protein kinase A is perhaps the prototype Ser/Thr kinase, which is activated by the second messenger cyclic AMP (cAMP). The PKA holoenzyme consists of two catalytic subunits complexed with two regulatory subunits. In addition, the regulatory subunits bind various AKAP proteins, which localize the holoenzyme to specific locations within the cell. Binding of cAMP to the regulatory subunits results in release of catalytic subunits, which phosphorylate an extremely broad range of substrates, including the transcription factor CREB. PKA activity in a lysate can be neutralized with the pseudosubstrate PKI.
Trade Name:
Chemicon (Millipore)
Format:
Affinity Purified
Antibody Type:
Polyclonal Antibody
Host:
Rabbit