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Ordering Information
- : AB1614
- : 100 µg
Anti-cAMP-Dependent Protein Kinase, Regulatory Subunit II-β, internal
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Mk, Ech | IH, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-cAMP-Dependent Protein Kinase, Regulatory Subunit II-β, internal
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes the regulatory subunit IIb of cyclic AMP-dependent protein kinase (PKA RIIbeta). Due to the sequence of the peptide selected, no cross-reactivity is seen with other PKA receptor isoforms.
Epitope:
Regulatory Subunit II-beta, internal
Immunogen:
Synthetic peptide coupled to KLH as an immune potentiator.
Species Reactivity:
- Human
- Mouse
- Monkey
- Echinoderms
Application Notes:
Western blotting 1:1,000
Immunohistochemistry 1:1,000
Optimal working dilutions must be determined by end user.
Immunohistochemistry 1:1,000
Optimal working dilutions must be determined by end user.
Presentation:
Peptide-affinity purified immunoglobulin. Liquid at 1 mg/mL containing 0.05% sodium azide and 50% glycerol.
Storage Conditions:
The undiluted antibody solution is stable for approximately 6 months when stored -20°C.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- PRKAR2B
- RII-BETA
- H_RG363E19.2
- WUGSC:H_RG363E19.2
- PRKAR2
Alternate Names:
PKA RIIbeta
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.
Key Applications:
- Immunohistochemistry
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P31323 # Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
SIZE: 418 amino acids; 46346 Da
SUBUNIT: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules.
TISSUE SPECIFICITY: Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
PTM: Phosphorylated by the activated catalytic chain.
SIMILARITY: SwissProt: P31323 ## Belongs to the cAMP-dependent kinase regulatory chain family. & Contains 2 cyclic nucleotide-binding domains.
SIZE: 418 amino acids; 46346 Da
SUBUNIT: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules.
TISSUE SPECIFICITY: Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
PTM: Phosphorylated by the activated catalytic chain.
SIMILARITY: SwissProt: P31323 ## Belongs to the cAMP-dependent kinase regulatory chain family. & Contains 2 cyclic nucleotide-binding domains.
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-cAMP-Dependent Protein Kinase, Regulatory Subunit II-β, internal
Antibody Type:
Polyclonal Antibody