Ordering Information
- : AB1690
- : 50 µL
Anti-Ubiquitin
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Eu, H | WB, IH, IH(P) | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-Ubiquitin
Trade Name:
Chemicon (Millipore)
Specificity:
Ubiquitin (76 amino acid, 8.5 kDa). Shows staining of neurofibrillary tangles, neurites in senile plaques and a subset of neuronal processes. No reactivity seen to tau, neurofilament or microtubule-associated proteins.
Immunogen:
Purified uboquitin covalently coupled to KLH using glutaraldehde.
Species Reactivity:
- Bovine
- Eukaryote
- Human
Application Notes:
Immunohistochemistry: 1:1,000-1:5,000. Tissue fixed in 10% formalin, Bouin's fixative for 1-2 days and embedded in paraffin, or unfixed frozen tissues may be used.
Immunoblotting: 1:1,000-1:5,000.
Optimal working dilutions must be determined by end user.
Immunoblotting: 1:1,000-1:5,000.
Optimal working dilutions must be determined by end user.
Presentation:
Neat rabbit antisera with 10 mM sodium azide.
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- UBC
- UBCEP1
- UBA80
- Ubiquitin.
- UBA52
- UBB
- HMG20
- UBCEP2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.
Key Applications:
- Western Blotting
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P62988 # Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous fonctions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.
SIZE: 76 amino acids; 8565 Da
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
SIMILARITY: SwissProt: P62988 ## Belongs to the ubiquitin family.
MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
SIZE: 76 amino acids; 8565 Da
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
SIMILARITY: SwissProt: P62988 ## Belongs to the ubiquitin family.
MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
Qty/Pk:
50 µL
Brand Family:
Chemicon
Format:
Serum
Host:
Rabbit
Protein/Isoform Description:
Ubiquitin is small protein that is covalently attached to particular lysine residues on other proteins, and a poly-ubiquitin chain develops on those proteins to mark them for degradation. Regulated degradation is critical for correct cell cycle progression, activation of NFκB, and p53 turnover. In addition, certain histones appear to be regulated by addition of a single ubiquitin molecule.
Product Name:
Anti-Ubiquitin
Antibody Type:
Polyclonal Antibody