Informazioni per l’ordine
Anti-cyclic AMP (cAMP)
| Specie riconosciute | Applicazioni fondamentali | Ospite | Forma | Tipo di anticorpo |
|---|---|---|---|---|
| A | ELISA, IH, RIA | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-cyclic AMP (cAMP)
Specificity:
Specific for cAMP with no cross reaction to other nucleotides at concentrations to 1 mM. Reactive with cAMP fixed in tissues or acetylated cAMP. Not reactive with free, non-acetylated, cAMP in RIA or EIA assays.
Key Applications:
- ELISA
- Immunohistochemistry
- Radioimmunoassay
Application Notes:
Immunohistochemistry: 1:1000 - 1:10,000, depending upon the detection method employed.
(See protocol on datasheet)
Radioimmunossay: Dilution of the antisera (50 μL) 1:3,000 will provide enough material to assay ~6000 tubes. Sensitivity is < 0.6 pg/50 μL in the acetylated cAMP RIA and the concentration of cyclic AMP producing 50% displacement from total binding is 1.5 ± .06 pg. Recommended protocol available upon request.
ELISA: 1:5,000 - 1:10,000. Acetylated cAMP or acetylated samples are required. Samples are acetylated as follows: In rapid succession, add 20:1 of triethylamine, followed by 10:1 acetic anhydride to each sample. Vortex. The procedure is performed at room temperature and may be done up to several hours prior to actually setting up the assay. Samples are diluted 50:1 with PBS prior to performing any assay.
(See protocol on datasheet)
Radioimmunossay: Dilution of the antisera (50 μL) 1:3,000 will provide enough material to assay ~6000 tubes. Sensitivity is < 0.6 pg/50 μL in the acetylated cAMP RIA and the concentration of cyclic AMP producing 50% displacement from total binding is 1.5 ± .06 pg. Recommended protocol available upon request.
ELISA: 1:5,000 - 1:10,000. Acetylated cAMP or acetylated samples are required. Samples are acetylated as follows: In rapid succession, add 20:1 of triethylamine, followed by 10:1 acetic anhydride to each sample. Vortex. The procedure is performed at room temperature and may be done up to several hours prior to actually setting up the assay. Samples are diluted 50:1 with PBS prior to performing any assay.
Species Reactivity:
All
Immunogen:
2'O-succinyl cyclic AMP conjugated to BSA
Control:
Widely expressed
Presentation:
Diluted antiSerum presented as liquid in PBS containing no preservatives.
Storage Conditions:
Stable upto 2 years at -80°C in undiluted aliquots from date of shipment. Avoid repeated freeze/thaw cycles.
Antibody Category:
Signaling
Antibody Sub-Category:
GPCR, cAMP/cGMP & Calcium Signaling
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Serum
Host:
Rabbit
Format Code:
Serum
Concentration:
Variable
Antibody Type:
Polyclonal Antibody
Purification Method:
UnPurified