Ordering Information
- : AB3272-50UL
- : 50 µL
- : 1 mg/mL (after reconstitution)
Product Images
Western blotting of rat kidney membranes (25 μg/lane)
1. AB3272 (1:1000)
2. AB3272, preincubated with the control peptide anti...
Anti-Aquaporin 1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | IH, WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-Aquaporin 1
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes Aquaporin 1.
SPECIES REACTIVITIES: The epitope specific for Aquaporin 1 is identical in human, rodent, bovine and only slightly different in frog. Reactivity with other species has not yet been tested.
SPECIES REACTIVITIES: The epitope specific for Aquaporin 1 is identical in human, rodent, bovine and only slightly different in frog. Reactivity with other species has not yet been tested.
Immunogen:
Highly purified peptide corresponding to residues 242-260 of human Aquaporin 1 (SwissProt Accession # P29972).
Species Reactivity:
- Human
- Rat
Species Reactivity Note:
The epitope specific for Aquaporin 1 is identical in rodent, bovine, sheep and only slightly different in frog (Rana esculenta) (18/19).
Application Notes:
Western blotting: 1:400-1:800 using ECL on rat kidney membranes.
Immunohistochemistry
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Immunohistochemistry
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Control:
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation:
Purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 0.025% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Storage Conditions:
Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- AQP1
- Aquaporin-CHIP
- MGC26324
- AQP-CHIP
- CO
- Aquaporin-1
- CHIP28
- AQP-1
Alternate Names:
AQP1; CHIP28
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunohistochemistry
- Western Blotting
Entrez Gene Summary:
Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This gene encodes an aquaporin which functions as a molecular water channel protein. It is a homotetramer with 6 bilayer spanning domains and N-glycosylation sites. The protein physically resembles channel proteins and is abundant in erythrocytes and renal tubes. The gene encoding this aquaporin is a possible candidate for disorders involving imbalance in ocular fluid movement.
UniProt Summary:
FUNCTION: SwissProt: P29972 # Forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
SIZE: 269 amino acids; 28526 Da
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
DOMAIN: SwissProt: P29972 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
MISCELLANEOUS: Pharmacologically inhibited by submillimolar concentrations of mercury.
SIZE: 269 amino acids; 28526 Da
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in a number of tissues including erythrocytes, renal tubules, retinal pigment epithelium, heart, lung, skeletal muscle, kidney and pancreas. Weakly expressed in brain, placenta and liver.
DOMAIN: SwissProt: P29972 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
MISCELLANEOUS: Pharmacologically inhibited by submillimolar concentrations of mercury.
Brand Family:
Chemicon
Product Name:
Anti-Aquaporin 1
Concentration:
1 mg/mL (after reconstitution)
Antibody Type:
Polyclonal Antibody
Qty/Pk:
50 µL
Format:
Purified
Host:
Rabbit