Ordering Information
- : AB3579
- : 100 µL
Product Images
Western Blotting Experiment Lysates prepared from Hek293T cells transfected with rat WT BimEL alone (lanes 1, 4), cotransfected with rat WT...
Anti-BimEL (pS69) human / (pS65) rat
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-BimEL (pS69) human / (pS65) rat
Trade Name:
Chemicon (Millipore)
Specificity:
Rat BimEL when phosphorylated at serine 65. Human BimEL when phosphorylated at serine 69. Mouse BimEL (100% conserved) has not been tested, but is expected to react.
Immunogen:
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of rat BimEL that contains serine 65 (serine 69 in the human sequence). The sequence is conserved in human and mouse.
Modifications:
Phosphorylation
Background Information:
Bim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family. There are three isoforms of Bim: BimEL, BimL, and BimS. Bim is involved in regulating the intrinsic mitochondrial apoptotic pathway by inducing cytochrome c release, which in turn, activates caspase-9 and then caspase-3. Bim also plays a critical role in central and peripheral deletion of T lymphocytes and in controlling B cell homeostasis and activation. BimEL, the long isoform of Bim, is a ~28 kDa protein that is predominantly expressed in T and B cells and is activated by ERK1/2 pathway. The activation of BimEL by ERK1/2 promotes its phosphorylation on serine 65 (serine 69 in human), targeting it for degradation via the proteasome.
Species Reactivity:
- Human
- Rat
Species Reactivity Note:
Mouse BimEL (100% homologous) has not been tested, but is expected to react.
Application Notes:
For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.
Control:
Hek293T co-expressing rat BimEL and active MEKK1 (MEKK1δ).
Quality Assurance:
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. The final product is generated by affinity chromatography using a BimEL-derived peptide that is phosphorylated at serine 65 (serine 69 in the human sequence).
Presentation:
Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide
Storage Conditions:
Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- BCL2L11
- BIM
- BOD
- BimEL
- BIM-beta6
- BimL
- BIM-beta7
- BAM
- BIM-alpha6
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified.
UniProt Summary:
FUNCTION: SwissProt: O43521 # Induces apoptosis. Isoform BimL is more potent than isoform BimEL.
SIZE: 198 amino acids; 22171 Da
SUBUNIT: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including Mcl-1, Bcl-2, Bcl-X(L), BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK (By similarity).
SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes (By similarity).
DOMAIN: SwissProt: O43521 The BH3 motif is required for Bcl-2 binding and cytotoxicity.
SIMILARITY:
SIZE: 198 amino acids; 22171 Da
SUBUNIT: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including Mcl-1, Bcl-2, Bcl-X(L), BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK (By similarity).
SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes (By similarity).
DOMAIN: SwissProt: O43521 The BH3 motif is required for Bcl-2 binding and cytotoxicity.
SIMILARITY:
Brand Family:
Chemicon
Protein/Isoform Description:
Bim is a pro-apoptotic member of the Bcl-2 protein family. Three Bim isoforms have been reported and designated as BimEL, BimL and BimS, all of which bind Bcl2 and induce apoptosis, with BimEL being the least cytotoxic. Bim is serine phosphorylated in lymphocytes, predominantly on the EL form, which potentiates its proapoptotic activity.
Product Name:
Anti-BimEL (pS69) human / (pS65) rat
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µL
Format:
Affinity Purified
Host:
Rabbit