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Ordering Information
- : AB3594-200UL
- : 200 µL
- : 0.3 mg/mL (after reconstitution)
Product Images
Western Blot Analysis:
Mouse Brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-AQUAPORIN 4 (1:500 d...
Anti-Aquaporin 4, C-terminus
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IH, WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-Aquaporin 4, C-terminus
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes Aquaporin 4 (AQP4, WCH4, Mercurial-Insensitive Water Channel).
Molecular Weight:
38 kDa
Epitope:
C-terminus
Immunogen:
GST fusion protein with residues 249-323 of rat Aquaporin 4 (SwissProt accession number P47863).
Background Information:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special proteins "aquaporins". Aquaporins, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. The water-specific channel Aquaporin 4 is located in various tissues including the gastrointestinal tract, kidney and brain. It is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. In CNS tissue, AQP4 localizes to astrocyte endfeet making it a useful structural marker.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Human, mouse, and rat. Reactivity with other species has not yet been tested. It is expected that the antibody may also react with bovine (71/75) and rabbit (64/75) due to sequence homology.
Application Notes:
Immunohistochemistry:
A previous lot of this antibody was used on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Western blotting:
1:500-1:1,000 using ECL on rat brain membranes.
Optimal working dilutions must be determined by the end user
A previous lot of this antibody was used on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Western blotting:
1:500-1:1,000 using ECL on rat brain membranes.
Optimal working dilutions must be determined by the end user
Control:
Included free of charge with the antibody is 120 µg of control antigen (lyophilized powder). Reconstitute with 100 µL of PBS. For positive control, in Western blot use 20 ng of protein per Minigel lane. For negative control, preincubate 3 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Quality Assurance:
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected AQUAPORIN 4 on 10 μg of Mouse Brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected AQUAPORIN 4 on 10 μg of Mouse Brain lysates.
Purification Method:
Immunoaffinity purified
Presentation:
Purified rabbit polyclonal lyophilized from buffer containing PBS, pH 7.4, containing 1% BSA, and 0.025% sodium azide. Reconstitute with 200 µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Storage Conditions:
Stable for up to 6 months at -20ºC from date of receipt. After reconstitution stable at -20ºC in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- AQP4
- MIWC
- WCH4
- AQP-4
- HMIWC2
- MGC22454
Alternate Names:
Mercurial-Insensitive Water Channel
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunohistochemistry
- Western Blotting
Entrez Gene Summary:
This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
UniProt Summary:
FUNCTION: SwissProt: P55087 # Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
SIZE: 323 amino acids; 34830 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Brain - muscle > heart, kidney, lung, and trachea.
DOMAIN: SwissProt: P55087 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. ...
SIZE: 323 amino acids; 34830 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Brain - muscle > heart, kidney, lung, and trachea.
DOMAIN: SwissProt: P55087 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family. ...
Brand Family:
Chemicon
Product Name:
Anti-Aquaporin 4, C-terminus
Concentration:
0.3 mg/mL (after reconstitution)
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 µL
Format:
Purified
Host:
Rabbit