Ordering Information
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Western Blot Analysis:
Mouse Brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-AQUAPORIN 4 (1:500 di...
Anti-Aquaporin 4, C-terminus
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IH, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P55087 # Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
SIZE: 323 amino acids; 34830 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Brain - muscle > heart, kidney, lung, and trachea.
DOMAIN: SwissProt: P55087 Aquaporins contain two tandem repeats each containing three membrane-spanning domains an ... see more »
SIZE: 323 amino acids; 34830 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Brain - muscle > heart, kidney, lung, and trachea.
DOMAIN: SwissProt: P55087 Aquaporins contain two tandem repeats each containing three membrane-spanning domains an ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Description:
Anti-Aquaporin 4, C-terminus
Background Information:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special proteins "aquaporins". Aquaporins, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. The water-specific channel Aquaporin 4 is located in various tissues including the gastrointestinal tract, kidney and brain. It is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. In CNS tissue, AQP4 localizes to astrocyte endfeet making it a useful structural marker.
Alternate Names:
AQP4; WCH4; Mercurial-Insensitive Water Channel
Specificity:
Recognizes aquaporin 4. On western blots of rat brain membranes, the antibody detects a band migrating at approximately 38 kDa corresponding to aquaporin 4.
Key Applications:
- Immunohistochemistry
- Western Blotting
Application Notes:
Western blotting: 1:500 on rat brain membranes.
Immunohistochemistry
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Immunohistochemistry
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Species Reactivity:
- Human
- Mouse
- Rat
Immunogen:
GST fusion protein with residues 249-323 of rat Aquaporin 4 (SwissProt accession number P47863). It is expected that the antibody may also react with mouse (73/75), bovine (71/75), human (69/75) and rabbit (64/75) due to sequence homology.
Epitope:
C-terminus
Molecular Weight:
38 kDa
Control:
CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen (lyophilized powder). Reconstitute with 100 μL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 3 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation:
Affinity Purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, and 0.025% sodium azide. Reconstitute with 50 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Storage Conditions:
Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Gene Symbol:
- AQP4
- MIWC
- WCH4
- AQP-4
- aquaporin-4
- HMIWC2
- Aquaporin-4
- MGC22454
Antibody Category:
Neuroscience
Antibody Sub-Category:
Ion Channels & Transporters
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Therapeutic Areas:
Inflammation
Format Code:
APur
Concentration:
0.3 mg/mL (after reconstitution)
Antibody Type:
Polyclonal Antibody
Format:
Affinity Purified
Host:
Rabbit
Purification Method:
Immunoaffinity Purified