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Western Blot Analysis:
Y79 lysates were resolved by electrophoresis, transferred to PVDF and probed with anti-OSTERIX (1:1000). Proteins were vi...
Anti-OSTERIX;100 µg
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-OSTERIX;100 µg
Specificity:
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptided selected from human OSTERIX
Molecular Weight:
45 kDa
Epitope:
C-terminus
Immunogen:
Synthetic human OSTERIX peptide, a.a. 412-427.
Background Information:
OSTERIX, or SP7, is a zinc finger-containing, osteoblast-specific transcription factor that is distinctly expressed in all developing bones, and is a key requirement for the differentiation of pre-osteoblasts into fully functional osteoblasts. While research on the actual functional mechanisms of OSTERIX is still on-going, current findings indicate that OSTERIX expression is induced by BMP-2 and Dlx5, and operates downstream of RUNX2, supporting its role in mesenchymal stem cell differentiation. Further studies also indicate that the OSTERIX transcription factor may also play a role in negatively regulating the Wnt/β-catenin signaling pathways and the proliferation of osteroblast cell types during embryonic osteogenesis.
Species Reactivity:
Human
Species Reactivity Note:
Reacts with human. Comparison of sequence homology suggests that this antibody may also react with mouse, rat, equine, and bovine; however, reactivity with other species has not been tested.
Application Notes:
Western Blotting:
Recommended working dilutions are 1:1000.
Recommended working dilutions are 1:1000.
Control:
Y79 lysates.
Quality Assurance:
Routinely evaluated by Western blot on Y79 lysates.
Purification Method:
Peptide affinity purified
Presentation:
Purified rabbit polyclonal in buffer containing PBS with 0.09% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- MGC126598
- OSX
- osterix
Alternate Names:
- Sp7 transcription factor
- Zinc finger protein osterix
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
SP7 is a C2H2-type zinc finger transcription factor of the SP gene family and a putative master regulator of bone cell differentiation (Gao et al., 2004 [PubMed 15474293]).[supplied by OMIM]
UniProt Summary:
FUNCTION: Transcriptional activator essential for osteoblast differentiation. Binds to Sp1 and EKLF consensus sequences and to other G/C-rich sequences (By similarity).
LOCATION: Nucleus (By similarity).
SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family.
SIMILARITY: Contains 3 C2H2-type zinc fingers.
LOCATION: Nucleus (By similarity).
SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family.
SIMILARITY: Contains 3 C2H2-type zinc fingers.
Stem Cell Type:
Mesenchymal Stem Cells
Stem Cell Workflow Stage:
Characterization
Product Name:
Anti-OSTERIX;100 µg
Concentration:
0.5 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µg
Format:
Affinity Purified
Host:
Rabbit