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Ordering Information
- : AB3854
- : 200 µL
Anti-UbcH1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-UbcH1
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes UbcH1 (E2-25K).
Immunogen:
Full length, highly purified, UbcH1 protein.
Background Information:
Unique among ubiquitin conjugating enzymes, E2-25K (UbcH2) catalyses the synthesis of poly-Ub chains linked via K48-G76 isopeptide bonds in vitro. It has been observed to form cyclic polyubiquitin and to add NEDD8 to poly-Ub chains. E2-25K functions only with mammalian E1.
Species Reactivity:
Human
Application Notes:
Western blot: 1:100-1:1000
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Presentation:
Protein A purified immunoglobulin in liquid PBS, pH 7.4, with 20% glycerol.
Storage Conditions:
Maintain at -70°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. May be stored at 2-8°C for up to 1 month.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- UBE2E1
- UbcH6
- UBCH6
- EC 6.3.2.19
Alternate Names:
E2-25K; E2 Ubiquitin-conjugating Enzyme
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P51965 # Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.
SIZE: 193 amino acids; 21404 Da
SUBUNIT: Interacts with RNF14.
SIMILARITY: SwissProt: P51965 ## Belongs to the ubiquitin-conjugating enzyme family.
SIZE: 193 amino acids; 21404 Da
SUBUNIT: Interacts with RNF14.
SIMILARITY: SwissProt: P51965 ## Belongs to the ubiquitin-conjugating enzyme family.
Qty/Pk:
200 µL
Brand Family:
Chemicon
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-UbcH1
Antibody Type:
Polyclonal Antibody