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Ordering Information
- : AB3855
- : 200 µL
Anti-UbcH2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-UbcH2
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes UbcH2 (E2-14K).
Immunogen:
Full length, highly purified, UbcH2 protein.
Background Information:
UbcH2 is the human homolog to the yeast DNA repair gene RAD6 and is inducible by DNA damaging agents. E2-14K is associated with cancer-induced cachexia and the degradation of "N-end rule" substrates. E2-14K mRNA level in muscle has also been correlated with sepsis. Moreover, c-Jun and c-Fos transcription factors have been shown to be ubiquitinated by an E2-14K-mediated event.
Species Reactivity:
Human
Application Notes:
Western blot: 1:100-1:1000
Immunoprecipitation
Optimal working dilutions must be determined by end user.
Immunoprecipitation
Optimal working dilutions must be determined by end user.
Presentation:
Protein A purified immunoglobulin in PBS, pH 7.4 and 20% glycerol.
Storage Conditions:
Maintain at -70°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. May be stored at 2-8°C for up to 1 month.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- UBE2H
- E2-20K
- UBCH
- UBC8
- UbcH2
- UBCH2
- EC 6.3.2.19
Alternate Names:
E2-14K; Ubiquitin Carrier Protein E2
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
Key Applications:
- Immunoprecipitation
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P62256 # Catalyzes the covalent attachment of ubiquitin to other proteins. Capable, in vitro, to ubiquitinate histone H2A.
SIZE: 183 amino acids; 20655 Da
SIMILARITY: SwissProt: P62256 ## Belongs to the ubiquitin-conjugating enzyme family.
SIZE: 183 amino acids; 20655 Da
SIMILARITY: SwissProt: P62256 ## Belongs to the ubiquitin-conjugating enzyme family.
Qty/Pk:
200 µL
Brand Family:
Chemicon
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-UbcH2
Antibody Type:
Polyclonal Antibody