Content Loading...
Content Loading...
Ordering Information
- : AB3856
- : 200 µL
Anti-UbcH3
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-UbcH3
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes UbcH3 (Cdc34).
Immunogen:
Full length, highly purified, UbcH3 protein.
Background Information:
UbcH3 is an essential gene for viability in yeast. Cdc34 mediates the transition from G1 to S-phase of the cell cycle by degrading the S-phase cyclin/CDK inhibitor SIC1 in yeast and p27(KIP1) in human. Cdc34 is also known to interact with the Skp1/Cdc53/F-box (SCF) ubiquitin ligase subunit Cul1 to degrade the NF-kB inhibitor IkBa in a phosphorylation-dependent manner.
Species Reactivity:
Human
Application Notes:
Western blot: 1:100-1:1000
Immunoprecipitation
Optimal working dilutions must be determined by end user.
Immunoprecipitation
Optimal working dilutions must be determined by end user.
Presentation:
Liquid in PBS, pH 7.4.
Storage Conditions:
Maintain at -70°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. May be stored at 2-8°C for up to 1 month.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CDC34
- UBE2R1
- UBC3
- E2-CDC34
- EC 6.3.2.19
Alternate Names:
Cdc34
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication.
Key Applications:
- Immunoprecipitation
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P49427 # Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in degradation of katenin.
SIZE: 236 amino acids; 26737 Da
SUBUNIT: Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. When phosphorylated, interacts with beta-TrCP (BTRC).
SIMILARITY: SwissProt: P49427 ## Belongs to the ubiquitin-conjugating enzyme family.
SIZE: 236 amino acids; 26737 Da
SUBUNIT: Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. When phosphorylated, interacts with beta-TrCP (BTRC).
SIMILARITY: SwissProt: P49427 ## Belongs to the ubiquitin-conjugating enzyme family.
Qty/Pk:
200 µL
Brand Family:
Chemicon
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-UbcH3
Antibody Type:
Polyclonal Antibody