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Ordering Information
- : AB3857
- : 200 µL
Anti-UbcH5
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-UbcH5
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes UbcH5.
Immunogen:
Full length, highly purified, UbcH5A protein.
Background Information:
UbcH5s are human homologs of the yeast UBC4/5 family and play many important regulatory roles in inflammation and cancer. UbcH5 mediates the degradation of a myriad of short-lived regulatory proteins including the tumor suppressor p53 in the presence of E6/E6-AP or MDM2, the c-Fos transcription factor, and the signal-induced ubiquitination of IkBalpha, as well as the processing of NF-kB1precursor, p105. This antibody recognizes UbcH5 isoforms A, B and C.
Species Reactivity:
Human
Application Notes:
Western blot: 1:100-1:1000
Immunoprecipitation
Optimal working dilutions must be determined by end user.
Immunoprecipitation
Optimal working dilutions must be determined by end user.
Presentation:
Protein A purified immunoglobulin in PBS, pH 7.4 containing 0.02% sodium azide and 20% glycerol.
Storage Conditions:
Maintain at -70°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. May be stored at 2-8°C for up to 1 month.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- UBE2D1
- UBCH5
- UBCH5A
- UbcH5A
- E2(17)KB1
- SFT
- E2
- UbcH5
- UBC4/5
- EC 6.3.2.19
Alternate Names:
E2-F1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
Key Applications:
- Immunoprecipitation
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P62837 # Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53.
SIZE: 147 amino acids; 16735 Da
SUBUNIT: Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex.
SIMILARITY: SwissProt: P62837 ## Belongs to the ubiquitin-conjugating enzyme family.
SIZE: 147 amino acids; 16735 Da
SUBUNIT: Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex.
SIMILARITY: SwissProt: P62837 ## Belongs to the ubiquitin-conjugating enzyme family.
Qty/Pk:
200 µL
Brand Family:
Chemicon
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-UbcH5
Antibody Type:
Polyclonal Antibody