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Ordering Information
- : AB5032
- : 50 µg
- : 1 mg/mL
Product Images
Rabbit anti-NCAM (Catalogue No. AB5032) shows strong staining of the condensing rudiments of the mouse peripheral nervous system.
Western blot of neuraminidase-treated mouse brain membrane, Triton X-100 extract, using Rabbit anti-NCAM (Catalog Number AB5032) illustrating major ba...
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Anti-Neural Cell Adhesion Molecule
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, H, M, R | ELISA, IF, IH, FUNC, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Neural Cell Adhesion Molecule
Trade Name:
Chemicon (Millipore)
Specificity:
Neural Cell Adhesion Molecule (NCAM). MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.
Molecular Weight:
MW is 200-250 kDa; when treated with Neuraminidase, 140-180 kDa. A breakdown product of 160 kDa may also be visible.
Immunogen:
Highly purified chicken NCAM
Background Information:
The Immunoglobulin superfamily of cell adhesion molecules is related to the immunoglobulins proteins by structure. The family contains over a 100 members, which include ICAM (intercellular adhesion molecule), NCAM (neural cell adhesion molecule) and NgCAM (neural-glial cell adhesion molecule). Both NCAM and NgCAM are calcium independent adhesion molecules. NCAM, the first CAM to be characterized, is expressed on all neurons from very early in development. It binds homophilically to NCAM on adjacent cells and has a variety of functions during neural development. One of these important functions is to hold developing neurites together during neuron outgrowth and formation of neural connections. The adhesive properties of NCAM are modulated during development and plasticity by the presence of the regulated carbohydrate, polysialic acid (PSA). The post-translational addition of alpha-2,8-linked PSA (NCAM-PSA) appears to contribute to synaptic plasticity during learning and memory formation and to the mechanisms of nerve regeneration.
Species Reactivity:
- Chicken
- Human
- Mouse
- Rat
Species Reactivity Note:
Mouse. Expected to cross-react with Chicken, Human, and rat.
Application Notes:
Immunohistochemistry:
A previous lot was shown to work with a wide range of fixatives. Will detect chicken NCAM using a concentration of 1 μg/mL and mouse NCAM using a concentration of 2 μg/mL.
Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.
Function blocking:
A previous lot of this antibody was used in Function blocking.
ELISA:
0.3 μg/mL of a previous lot was used.
Optimal working dilutions must be determined by end user.
A previous lot was shown to work with a wide range of fixatives. Will detect chicken NCAM using a concentration of 1 μg/mL and mouse NCAM using a concentration of 2 μg/mL.
Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.
Function blocking:
A previous lot of this antibody was used in Function blocking.
ELISA:
0.3 μg/mL of a previous lot was used.
Optimal working dilutions must be determined by end user.
Control:
Neuraminidase-treated mouse brain membrane.
Quality Assurance:
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected NCAM on 10 μg of mouse brain lysate.
Western Blot Analysis: 1:1000 dilution of this lot detected NCAM on 10 μg of mouse brain lysate.
Purification Method:
Immunoaffinity purified
Presentation:
Purified rabbit polyclonal in buffer containing PBS containing no preservatives.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CD56
- NCAM
- MSK39
- NCAM1
Alternate Names:
- CD56 antigen
- antigen recognized by monoclonal antibody 5.1H11
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunofluorescence
- Immunohistochemistry
- Affects Function
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P13592 # This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.| P13591 # This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
SIZE: 761 amino acids; 83770 Da
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
DOMAIN: SwissProt: P13592
SIMILARITY: SwissProt: P13592 ## Contains 2 fibronectin type-III domains. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. | P13591 ## Contains 2 fibronectin type-III domains. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
SIZE: 761 amino acids; 83770 Da
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
DOMAIN: SwissProt: P13592
SIMILARITY: SwissProt: P13592 ## Contains 2 fibronectin type-III domains. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. | P13591 ## Contains 2 fibronectin type-III domains. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Qty/Pk:
50 µg
Brand Family:
Chemicon
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-Neural Cell Adhesion Molecule
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody