Anti-Choline Acetyltransferase
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | ELISA, IH | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Choline Acetyltransferase
Trade Name:
Chemicon (Millipore)
Specificity:
Choline acetyltranferase. The antibody will stain cholinergic neurons in the rat central and peripheral nervous systems.
Immunogen:
A 22 amino acid synthetic peptide from porcine ChAT, coupled to KLH. The peptide sequence is GLFSSYRLPGHTQDTLVAQKSS. Due to sequence similarity, the antibody is expected to react with pig, mouse primate and human ChAT.
Species Reactivity:
Rat
Application Notes:
Immunohistochemistry: 1:2,000-1:4,000 in rat basal forebrain.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Presentation:
Affinity purified immunoglobulin. Lyophilized. Contains no preservative. Reconstitute with 20 μL of sterile distilled water. Centrifure to remove any insoluble material.
Storage Conditions:
Maintain lyophilized material dry at -70°C for up to 12 months after date of receipt. After reconstitution, maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CHAT
- ChAT
- CMS1A2
- CHOACTase
- CMS1A
- EC 2.3.1.6
Alternate Names:
ChAT
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.
Key Applications:
- ELISA
- Immunohistochemistry
UniProt Summary:
FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
SIZE: 748 amino acids; 82568 Da
DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
SIZE: 748 amino acids; 82568 Da
DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
Qty/Pk:
20 µg
Brand Family:
Chemicon
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-Choline Acetyltransferase
Antibody Type:
Polyclonal Antibody