Ordering Information
- : AB5060
- : 50 µL
- : Variable
Product Images
Rabbit anti-Substance P Receptor [NK-1] (Catalog Number AB5060). SPR staining is shown in red. Staining of rat spinal cord.
Rabbit anti-Substance P Receptor (Cat. No. AB5060). Localization of Substance P Receptor in rat brain. Photo courtesey of Sergiy Tadtayev, NPIMR, Harr...
Anti-Substance P Receptor, pain
Recommended Replacement for: AB5800| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Gp, M, R | IF, IH, WB | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-Substance P Receptor, pain
Replaces:
AB5800
Trade Name:
Chemicon (Millipore)
Specificity:
Specific for the Substance P Receptor (NK-1).
Molecular Weight:
35 kDa
Epitope:
C terminus
Immunogen:
Synthetic peptide that corresponds to a 23 amino acid sequence (385-407) of the COOH terminus of the rat Substance P Receptor (NK-1).
Background Information:
Substance P is a tachykinin family neuropeptide that functions as a neurotransmitter and neuromodulator. In the CNS, Substance P is involved in the regulation of mood, anxiety, sleep, saiety, reinforcement, respiratory rhythm and pain processing. In nociception, the neuropeptide is released at local spinal cord synapses. Recent research now shows substance P is potentially involved in endothelial cell differentiation as well as diverse roles in neuroinflammatory response. The endogenous receptor for Substance P is neurokinin 1 receptor (NK1 receptor), which belongs to the tachykinin receptor sub-family of GPCRs.
Species Reactivity:
- Guinea Pig
- Mouse
- Rat
Species Reactivity Note:
Rat, mouse and guinea pig.
Application Notes:
Immunohistochemistry:
A previous lot was used at 1:100-1:1,000 dilution.
Western Blot Analysis:
A previous lot was used at 1:500 dilution.
Optimal working dilutions must be determined by the end user.
A previous lot was used at 1:100-1:1,000 dilution.
Western Blot Analysis:
A previous lot was used at 1:500 dilution.
Optimal working dilutions must be determined by the end user.
Control:
Spinal Cord, brain membrane lysate
Quality Assurance:
Evaluated by western blot on Mouse brain membrane lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected NK-1R on 10 μg of Mouse brain membrane lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected NK-1R on 10 μg of Mouse brain membrane lysates.
Purification Method:
Unpurified
Presentation:
Rabbit polyclonal serum in buffer containing liquid with no preservatives.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- TAC1
- NK2
- NKA
- NKNA
- Hs.2563
- PPT
- TAC2
Alternate Names:
NK-1 Receptor
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunofluorescence
- Immunohistochemistry
- Western Blotting
Entrez Gene Summary:
This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Summary:
FUNCTION: SwissProt: P20366 # Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
SIZE: 129 amino acids; 15003 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P20366 ## Belongs to the tachykinin family.
SIZE: 129 amino acids; 15003 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P20366 ## Belongs to the tachykinin family.
Brand Family:
Chemicon
Product Name:
Anti-Substance P Receptor, pain
Concentration:
Variable
Antibody Type:
Polyclonal Antibody
Qty/Pk:
50 µL
Format:
Serum
Host:
Rabbit